Q1GYH7 (AROE_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Mfla_2445

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021303
Regions
	Nucleotide binding	126 – 130	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1GYH7 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: C4546DCC2F4C7890	FASTA	270	28,904
	10 20 30 40 50 60 MDRYAVIGHP VEHSKSPLIH AAFAQQTGQQ LEYGKVQAPL DGFVAIVTDL QEKGYRGVNV 70 80 90 100 110 120 TVPFKFEAYS LATELTPRAR DAGAVNTLTF TDGAIHGDNT DGVGLVRDIL QNLAFTLAGK 130 140 150 160 170 180 RVLLLGAGGA AEGVLAPLLE ASPLSLCIAN RTVDKAEAMA ARVRPGQTSL DARGFDALAD 190 200 210 220 230 240 QEFDVVINAT SSGLHNERLP LPSGLFAADA LAYDMMYGRE TPFMRYAREE GASRIADGLG 250 260 270 MLVEQAAEAF YIWRRVRPDT APVMAALRAA

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE50710.1.
RefSeq	YP_546551.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1GYH7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1GYH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4001541.
GenomeReviews	Gene locus Mfla_2445 in contig CP000284_GR.
KEGG	mfa:Mfla_2445.
NMPDR	fig|265072.7.peg.2475.
PATRIC	32270713. VBIMetFla97085_2565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	VVFGNPI.
PhylomeDB	Q1GYH7.
ProtClustDB	CLSK2529799.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_2445-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_METFK

Accession

Primary (citable) accession number: Q1GYH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program