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Q1GYC1

- LIPA_METFK

UniProt

Q1GYC1 - LIPA_METFK

Protein

Lipoyl synthase

Gene

lipA

Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMFLA265072:GHWJ-2560-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Mfla_2501
    OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
    Taxonomic identifieri265072 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
    ProteomesiUP000002440: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Lipoyl synthasePRO_0000325276Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi265072.Mfla_2501.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1GYC1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiFERIATN.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1GYC1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEITERIVP PPRKVAGVKE SSAEKMARIP IKIIPMPAMR KPEWIRMKVP    50
    DSARFREIKQ VLRENNLHTV CEEASCPNIG ECFSGGTATF MILGDICTRR 100
    CPFCDVSHGK PLPPDANEPE NLGRTIAQMR LKYVVITSVD RDDLRDGGAQ 150
    HFVDCIAAVR AHSPQIKVEI LVPDFRGRLD RAIHILKAAP PDVMNHNLET 200
    VPRLYKEARP GSDYQNSLDL LKEFGKLHPE VPTKSGLMLG LGETDEEILD 250
    VMRDLRAHNV TMLTLGQYLQ PSPHHLPVKR FVTPQRFAEF ERQALAMGFT 300
    HAACGPMVRS SYHADHQAQQ AGVNF 325
    Length:325
    Mass (Da):36,464
    Last modified:June 27, 2006 - v1
    Checksum:iAFE66D27AED7C95E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000284 Genomic DNA. Translation: ABE50766.1.
    RefSeqiWP_011480719.1. NC_007947.1.
    YP_546607.1. NC_007947.1.

    Genome annotation databases

    EnsemblBacteriaiABE50766; ABE50766; Mfla_2501.
    GeneIDi4001597.
    KEGGimfa:Mfla_2501.
    PATRICi32270825. VBIMetFla97085_2621.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000284 Genomic DNA. Translation: ABE50766.1 .
    RefSeqi WP_011480719.1. NC_007947.1.
    YP_546607.1. NC_007947.1.

    3D structure databases

    ProteinModelPortali Q1GYC1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 265072.Mfla_2501.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE50766 ; ABE50766 ; Mfla_2501 .
    GeneIDi 4001597.
    KEGGi mfa:Mfla_2501.
    PATRICi 32270825. VBIMetFla97085_2621.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi FERIATN.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci MFLA265072:GHWJ-2560-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KT / ATCC 51484 / DSM 6875.

    Entry informationi

    Entry nameiLIPA_METFK
    AccessioniPrimary (citable) accession number: Q1GYC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3