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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861MagnesiumUniRule annotation
Metal bindingi492 – 4921MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMFLA265072:GHWJ-77-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Mfla_0071
OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Taxonomic identifieri265072 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
ProteomesiUP000002440: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Polyribonucleotide nucleotidyltransferasePRO_0000329712Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi265072.Mfla_0071.

Structurei

3D structure databases

ProteinModelPortaliQ1GXD2.
SMRiQ1GXD2. Positions 617-690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KHUniRule annotationAdd
BLAST
Domaini622 – 69069S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1GXD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAIKKTITY GQHELTLETG EIARQADGAV MVSYGDTVVL VSVVGKREVK
60 70 80 90 100
EGQDFFPLTV DYQEKTYAAG KIPGGFFKRE GRPSEKETLT SRLIDRPLRP
110 120 130 140 150
LFPEAFYNEV QVVATVMSSD PEIDADIPAI IGASAALAIS GIPFYGPIGA
160 170 180 190 200
ARVGYINGEY VLNPTASQLK ETALDLVVAA TESAVLMVES EAKELPEDVM
210 220 230 240 250
LGSVVYGHEQ MQIVISAINE LAAEVGKEPW DWAPPEPNTE LIAKVEALAG
260 270 280 290 300
ADINEAYKIK SKSARSSKLD EIRSRVLGEL ITETTSTSEA NEIKSIFHNL
310 320 330 340 350
EAKVVRSQIL NGEPRIDGRD TRTVRPISIR TGVLPRTHGS ALFTRGETQA
360 370 380 390 400
LVVATLGTGR DEQIIDALQG EYADRFMLHY NMPPYATGET GRVGTPKRRE
410 420 430 440 450
IGHGRLAKRA LLAVLPSQEE FGYTIRVVSE ITESNGSSSM ASVCGGCLSL
460 470 480 490 500
LDAGVPLKAH VAGIAMGLIK EGNRVAVLTD ILGDEDHLGD MDFKVAGTES
510 520 530 540 550
GITALQMDIK ITGITAEIMR VALSQAKEGR LHILGLMKEA VGEQPQELSE
560 570 580 590 600
FAPRIITMKI NPEKIRDVIG KGGAVIRALT EETGTTIDIE EDGTIKIGCT
610 620 630 640 650
SAEAGEEAKK RIEAITAEVE VGQVYDGTVL KLLDFGAIVS LLPGKDGLLH
660 670 680 690 700
ISQIAHQRVN AVADFLKEGQ VVKVKVLEVD DKGRVRLSAK ALIEAPSAEA
710
PEEPAVAATG ADQQ
Length:714
Mass (Da):76,634
Last modified:June 27, 2006 - v1
Checksum:iB66742F931E4FBEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000284 Genomic DNA. Translation: ABE48342.1.
RefSeqiYP_544183.1. NC_007947.1.

Genome annotation databases

EnsemblBacteriaiABE48342; ABE48342; Mfla_0071.
GeneIDi3999090.
KEGGimfa:Mfla_0071.
PATRICi32265641. VBIMetFla97085_0072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000284 Genomic DNA. Translation: ABE48342.1.
RefSeqiYP_544183.1. NC_007947.1.

3D structure databases

ProteinModelPortaliQ1GXD2.
SMRiQ1GXD2. Positions 617-690.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265072.Mfla_0071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE48342; ABE48342; Mfla_0071.
GeneIDi3999090.
KEGGimfa:Mfla_0071.
PATRICi32265641. VBIMetFla97085_0072.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciMFLA265072:GHWJ-77-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT / ATCC 51484 / DSM 6875.

Entry informationi

Entry nameiPNP_METFK
AccessioniPrimary (citable) accession number: Q1GXD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 27, 2006
Last modified: February 4, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.