Q1GTU5 (Q1GTU5_SPHAL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Sala_1211

Organism

Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]

Taxonomic identifier

317655 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingopyxis

Protein attributes

Sequence length	849 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q1GTU5 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: A57DFE0770E1C87C	FASTA	849	93,069
	10 20 30 40 50 60 MQLVIVESPA KAKTIEKYLG RDFKVLASYG HVRDLPPKDG SVDPDDGFAM QWQLYPDKAK 70 80 90 100 110 120 RLKEISDAAK GADRLILATD PDREGEAISW HVQELLRSKK ALPAAVDRVT FNAITKQAVT 130 140 150 160 170 180 DAMRHPRELD TDLIDAYRAR RALDYLVGFT LSPVLWRKLP GAKSAGRVQS VALRLVVERE 190 200 210 220 230 240 REIEAFVAQQ YWSVTGLFEM GGTPFKARLS RWRGDKIERL SITSEADARA AEADVKAGHF 250 260 270 280 290 300 TVDTVETKPL TRNPPPPFTT STLQQEAARK LGFSASHTMR IAQALYEDGA ITYMRTDGVQ 310 320 330 340 350 360 MDGSAISAAR KAIATRYDGG YVPDKPRQYQ TKAKNAQEAH EAIRPTDFGK DKVGGGDHAR 370 380 390 400 410 420 LYELIWKRAL ASQMASARLE RTSVELSDGT GKTTLRATGQ VVKFPGFLAL YDEGRDDAED 430 440 450 460 470 480 EDARLLPAMA KGDAPAKTGV EVERHETQPP PRYSEASLVK KMEELGIGRP STYASILQVL 490 500 510 520 530 540 KDRDYVRVEK NRFIPEESGR LLTAFLERFF ERYVGYDFTA ELEEELDDVS GGRAQWQAVL 550 560 570 580 590 600 ERFWRDFKPR TGEVMEQKPS EITAALDEFL GPYLFPDKSD GSDPRLCPNC GTGRLALRGG 610 620 630 640 650 660 KFGPFIACSN YPDCKFTRKF AQSGGNGEAD NGPETLGTDP ESGLEVTKRS GRFGPYIQLG 670 680 690 700 710 720 EGSEAKRSSI PKDIPAEDFD LDYALRLLSL PREVGVHPES GKPIMAGLGR YGPYLLHDGK 730 740 750 760 770 780 YAKLGGTAEI FDIGMNAAVV RIAEAANGGG RGRAKAEPLN TFGPHPTSGG EMKLMEGRYG 790 800 810 820 830 840 PYVTDGTTNA TLPKTTDPAT LTMEEAIALI DARAAKGPVK GKKKAAPKKK AAAKKAAAKE APARKTAAE

References

[1]

"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000356 Genomic DNA. Translation: ABF52927.1.
RefSeq	YP_616260.1. NC_008048.1.
3D structure databases
ProteinModelPortal	Q1GTU5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4080706.
GenomeReviews	Gene locus Sala_1211 in contig CP000356_GR.
KEGG	sal:Sala_1211.
NMPDR	fig|317655.9.peg.1145.
PATRIC	23691615. VBISphAla23391_1247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	SALA317655:SALA_1211-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q1GTU5_SPHAL

Accession

Primary (citable) accession number: Q1GTU5

Entry history

Integrated into UniProtKB/TrEMBL:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)