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Q1GTT5

- BIOB_SPHAL

UniProt

Q1GTT5 - BIOB_SPHAL

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Protein
Biotin synthase
Gene
bioB, Sala_1222
Organism
Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi186 – 1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi264 – 2641Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSALA317655:GHHY-1229-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Sala_1222
OrganismiSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
Taxonomic identifieri317655 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingopyxis
ProteomesiUP000006578: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Biotin synthaseUniRule annotation
PRO_0000381640Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi317655.Sala_1222.

Structurei

3D structure databases

ProteinModelPortaliQ1GTT5.
SMRiQ1GTT5. Positions 2-319.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1GTT5-1 [UniParc]FASTAAdd to Basket

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MRTDWTREEI AALFDLPFDE LMWEAQGVHR RHHARGEIQL CTLLSIKTGG    50
CVEDCGYCSQ SAHADTGLKA TKLMDVRAVL QAAAQAKDAG SKRFCMGAAW 100
RNPKDRDMPA IVEMIEGVRA MGMETCMTLG MLSKEQAQVL GMAGLDYYNH 150
NIDTSPEHYG NVITTRSFQD RLDTLEEVRA AGINVCSGGI VGMGETRADR 200
VGFIHTLATL PEHPGSVPIN ALVPVKGTVL GDMLADTPLA KIDDIEFVRT 250
VAVARITMPK SMVRLSAGRE SMSEATQALC FMAGANSIFT GDKLLTTANA 300
GDNADAALFA KLGLRPMESE EPMRMEAAE 329
Length:329
Mass (Da):35,642
Last modified:June 27, 2006 - v1
Checksum:i87804FD031D59A5E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000356 Genomic DNA. Translation: ABF52937.1.
RefSeqiYP_616270.1. NC_008048.1.

Genome annotation databases

EnsemblBacteriaiABF52937; ABF52937; Sala_1222.
GeneIDi4080446.
KEGGisal:Sala_1222.
PATRICi23691640. VBISphAla23391_1259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000356 Genomic DNA. Translation: ABF52937.1 .
RefSeqi YP_616270.1. NC_008048.1.

3D structure databases

ProteinModelPortali Q1GTT5.
SMRi Q1GTT5. Positions 2-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 317655.Sala_1222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF52937 ; ABF52937 ; Sala_1222 .
GeneIDi 4080446.
KEGGi sal:Sala_1222.
PATRICi 23691640. VBISphAla23391_1259.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SALA317655:GHHY-1229-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13593 / LMG 18877 / RB2256.

Entry informationi

Entry nameiBIOB_SPHAL
AccessioniPrimary (citable) accession number: Q1GTT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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