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Q1GTH6 (TYSY_SPHAL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Sala_1332
OrganismSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]
Taxonomic identifier317655 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingopyxis

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Sequence caution

The sequence ABF53046.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000321484

Sites

Active site1921 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GTH6 [UniParc].

Last modified March 3, 2009. Version 2.
Checksum: 2CA7FE05EC3C05D0

FASTA31135,515
        10         20         30         40         50         60 
MADSIHYELQ YLDLMRRIWV DGDERVDRTG VGTRSLFGET MRFSLKDDAI PLLTTKRVYW 

        70         80         90        100        110        120 
KTALRELLWF LTGDTNIRPL VAQGVKIWTD WPLEKYRKAT GEAISATDFE ARIVADADFA 

       130        140        150        160        170        180 
AKWGDLGPVY GHQWVNWPRY EPAGGGLFRR ADHGYNQIAA LIDSLKNNPG SRRHIFTGWN 

       190        200        210        220        230        240 
VADLDRMALP PCHMTYQFHV RSDGGLSCLL FQRSCDLGLG FAFNIFEAAL LTRMVAEQCG 

       250        260        270        280        290        300 
LYAHEVVWTG GDVHLYLNHA ELVEQQLARV PEGKPRLRIL RRPPSIFEYR FEDFVVEDYA 

       310 
PQAHISAPVA V 

« Hide

References

[1]"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000356 Genomic DNA. Translation: ABF53046.1. Different initiation.
RefSeqYP_616379.1. NC_008048.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317655.Sala_1332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF53046; ABF53046; Sala_1332.
GeneID4081003.
KEGGsal:Sala_1332.
PATRIC23691882. VBISphAla23391_1376.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycSALA317655:GHHY-1343-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_SPHAL
AccessionPrimary (citable) accession number: Q1GTH6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways