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Q1GRY2 (DDL_SPHAL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sala_1878
OrganismSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]
Taxonomic identifier317655 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingopyxis

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341177

Regions

Domain104 – 304201ATP-grasp
Nucleotide binding131 – 18757ATP By similarity

Sites

Metal binding2551Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 2 By similarity
Metal binding2731Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GRY2 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 867191BFEA5D2EC2

FASTA30833,521
        10         20         30         40         50         60 
MSRGPWHVAV LMGGWSAERE VSLMSGKGVA DALESRGHKV TRIDMDRDVA LRLAEAKPDV 

        70         80         90        100        110        120 
VFNALHGVPG EDGTVQGMLD LMGFRYTHSG LVTSVIAIDK ELTKQALVPH GIPMPTGTMV 

       130        140        150        160        170        180 
DSESLFSVDP LPRPYVLKPV NEGSSVGVAI VRDDSNYGNP ISRDALGPWQ QFDRLLAEPF 

       190        200        210        220        230        240 
IKGRELTVAV LGDTALAVTE LRVKSGFYDY DAKYTDGLTE HVCPADVPDD VAQRMKDLAL 

       250        260        270        280        290        300 
QAHRLLGCKG ASRSDFRWDD EHGLAGIFLL EVNTQPGMTP LSLVPEQARA VGMDYAELVE 


RIVEEALT 

« Hide

References

[1]"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000356 Genomic DNA. Translation: ABF53590.1.
RefSeqYP_616923.1. NC_008048.1.

3D structure databases

ProteinModelPortalQ1GRY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317655.Sala_1878.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF53590; ABF53590; Sala_1878.
GeneID4082623.
KEGGsal:Sala_1878.
PATRIC23693046. VBISphAla23391_1944.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMALCRWIVE.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycSALA317655:GHHY-1907-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SPHAL
AccessionPrimary (citable) accession number: Q1GRY2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways