Q1GR67 (KATG_SPHAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Sala_2146

Organism

Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]

Taxonomic identifier

317655 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingopyxis

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Catalase-peroxidase HAMAP MF_01961		PRO_0000354937
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254) By similarity
	Cross-link	227 ↔ 254		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GR67 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 674F7F4DFC5A5A4C	FASTA	731	80,470
	10 20 30 40 50 60 MNDQTPIGSG CPVHQPGGVR SLLGRTNKDW WPDMLATEIL TPNGPSNPMG EDFDYAKAFK 70 80 90 100 110 120 SLDYYALKDD LKALMTDSQP WWPADYGHYG PFFIRMAWHA AGTYRTADGR GGANSGQQRF 130 140 150 160 170 180 APLDSWPDNG NLDKARRLLW PIKQKYGNKI SWADLFILAG NVAIESMGGP VFGFGGGRVD 190 200 210 220 230 240 VYEPERDIYW GSEDKWVNQG VQTRIDPAKG METIEGPLAA IQMGLIYVNP EGPQGNPHDD 250 260 270 280 290 300 EGMARDMKET FKRMAMNDEE TVALTAGGHT FGKAHGNGDP SLLGPAPAGS DLAAQGFGWV 310 320 330 340 350 360 SSHESGGIGE HAVTSGIEGA WTNTPREWTE NYFRLLFDYD YELVKSPAGA WQWQPINQKE 370 380 390 400 410 420 EDMAPAAWDP GIKVPTMMTT ADMALKRDPA YRAISERFRN DHEAFKDAFA RAWFKLTHRD 430 440 450 460 470 480 MGPKVRYLGP EVPDEDLIWQ DPIPAGTKPS DAEVQAVKDK IAASGLTVSQ LIKTAWASAS 490 500 510 520 530 540 TFRKSDFRGG ANGARVRLAP QKDWEVNEPA MLARVLDTLD GLRGSLSMAD AIVLGGVVGL 550 560 570 580 590 600 EKAIRDAGFN VAVPFTGGRG DATQEQTDVE SFEVMEPEAD AFRNYVGKKK LAVKVEEMML 610 620 630 640 650 660 DKASLLGLSV PEMTVLIGGL RVLGANHGER GHGHFTRRSG QLTNDFFVNL LDMTNVWKAV 670 680 690 700 710 720 EGSNDQEYVA TDRTTGGETW RATRADLIFG SNSELRAVAE VYAENGHEEK FVRDFVKAWT 730 KVMNADRFDL A

References

[1]

"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000356 Genomic DNA. Translation: ABF53855.1.
RefSeq	YP_617188.1. NC_008048.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4080143.
GenomeReviews	Gene locus Sala_2146 in contig CP000356_GR.
KEGG	sal:Sala_2146.
NMPDR	fig|317655.9.peg.2072.
PATRIC	23693616. VBISphAla23391_2224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	SALA317655:SALA_2146-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SPHAL

Accession

Primary (citable) accession number: Q1GR67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program