Q1GP96 (EFG_SPHAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Sala_2821

Organism

Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]

Taxonomic identifier

317655 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingopyxis

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Elongation factor G HAMAP MF_00054_B		PRO_0000263513
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	88 – 92	5	GTP By similarity
	Nucleotide binding	142 – 145	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GP96 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 2FEB8D85879F4D4F	FASTA	697	76,700
	10 20 30 40 50 60 MARSHPLERY RNFGIMAHID AGKTTTTERI LYYTGKSYKI GEVHDGAATM DWMEQEQERG 70 80 90 100 110 120 ITITSAATTC LWKADEGKGP EHRLNIIDTP GHVDFTIEVE RSLRVLDGAV AAFDGVAGVE 130 140 150 160 170 180 PQSETVWRQA DKYKVPRMCF INKLDRTGAN FYYCVQTIID RLGATPAVLY LPIGAESDFK 190 200 210 220 230 240 GLVDLVNERA IIWKDESLGA EFFYEDIPAD LADKAAEYRE KLVELAVEQD DEAMEAYLEG 250 260 270 280 290 300 NVPDVATLKK LIRKGTLNQA FVPVLCGSAF KNKGVQPLLD AVVDYLPSPL DIPDVQGINP 310 320 330 340 350 360 TTEQPDSRAT SDSAPLSMLA FKIMNDPFVG SLTFARIYSG TLTKGSYLNS VKDKKEKIGR 370 380 390 400 410 420 MLLMHANSRE DIEEAFAGDI VALAGLKETT TGDTLCASNA PIILERMEFP EPVIELSVEP 430 440 450 460 470 480 KTKADQEKMG IALSRLAAED PSFRVSTDHE SGQTIIKGMG ELHLDILVDR MKREFKVEAN 490 500 510 520 530 540 VGAPQVAYRE SLAKPVDVDY THKKQSGGSG QFGRVKVSVA PGERGSGITF IDEIKGGNIP 550 560 570 580 590 600 REYIPSVEKG MREAAENGHM IGFPIIDFEI RLTDGAYHDV DSSALAFEIA GRAAMREVAA 610 620 630 640 650 660 KAGIKLLEPV MKVEVVTPEE FMGDVIGDLN SRRGQIQGTD SRGNAQVVEA MVPLANMFGY 670 680 690 VNQLRSFTQG RAQYTMQFSH YEEVPNNVAE EVKAKMA

References

[1]

"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000356 Genomic DNA. Translation: ABF54526.1.
RefSeq	YP_617859.1. NC_008048.1.
3D structure databases
ProteinModelPortal	Q1GP96.
SMR	Q1GP96. Positions 4-692.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4080402.
GenomeReviews	Gene locus Sala_2821 in contig CP000356_GR.
KEGG	sal:Sala_2821.
NMPDR	fig|317655.9.peg.2686.
PATRIC	23695014. VBISphAla23391_2915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	SALA317655:SALA_2821-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_SPHAL

Accession

Primary (citable) accession number: Q1GP96

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program