Q1GNH3 (ARGB_SPHAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	Sala_3095

Organism

Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis) [Complete proteome] [HAMAP]

Taxonomic identifier

317655 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingopyxis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000264764
Regions
	Region	73 – 74	2	Substrate binding By similarity
Sites
	Binding site	95	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity
	Site	38	1	Transition state stabilizer By similarity
	Site	260	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GNH3 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: A4C0D1A2D5948AD3	FASTA	302	31,189
	10 20 30 40 50 60 MMTMADPSKM PDLLAKAETL VEALPYLQRY AGQTFVIKYG GHAMGDPEAQ RDFAEDVVLL 70 80 90 100 110 120 KAVGINPVVV HGGGPQIGAM LKQLGIESTF VGGLRVTDAA TAEVAEMVLA GKINKEIVGW 130 140 150 160 170 180 IAGLGGRAVG ISGKDANLVL AEKVKRSEAD PSSGIERHVD LGFVGEPVAV DPTILANLTN 190 200 210 220 230 240 DNFIPVVAPV ALGADGATYN INADTMAGAI AGALGAKRFF LLTDVAGVLD KSGALLTDLD 250 260 270 280 290 300 RAAIDALKAD DTITGGMIPK VETCVAAVDA GVEAAVILDG RIPHAMLLEI FTTRGAGTLI HR

References

[1]

"Complete sequence of chromosome of Sphingopyxis alaskensis RB2256." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Chertkov O., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Cavicchioli R., Robb F., Ertan H., Schut F., Ting L.M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13593 / LMG 18877 / RB2256.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000356 Genomic DNA. Translation: ABF54799.1.
RefSeq	YP_618132.1. NC_008048.1.
3D structure databases
ProteinModelPortal	Q1GNH3.
SMR	Q1GNH3. Positions 14-302.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4082831.
GenomeReviews	Gene locus Sala_3095 in contig CP000356_GR.
KEGG	sal:Sala_3095.
NMPDR	fig|317655.9.peg.2947.
PATRIC	23695570. VBISphAla23391_3190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	SALA317655:SALA_3095-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_SPHAL

Accession

Primary (citable) accession number: Q1GNH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program