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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621S-adenosyl-L-methionineUniRule annotation
Binding sitei87 – 871S-adenosyl-L-methionineUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Active sitei141 – 1411By similarity
Binding sitei141 – 1411S-adenosyl-L-methionineUniRule annotation
Binding sitei145 – 1451SubstrateUniRule annotation
Binding sitei177 – 1771SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSALA317655:GHHY-3182-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Sala_3140
OrganismiSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
Taxonomic identifieri317655 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingopyxis
ProteomesiUP000006578: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA (guanine-N(7)-)-methyltransferasePRO_0000288231Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi317655.Sala_3140.

Structurei

3D structure databases

ProteinModelPortaliQ1GNC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2194Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1GNC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAHKPGDPT TLNRLYGRSK GKPLRAGQQD LVDTLLPQIA VPLDGEVTAR
60 70 80 90 100
RLFGFDRPLH FEIGFGGGEH MAARADMLPD HGFIGAEPFI NGVAQALVHV
110 120 130 140 150
AGDHGQHPPL GNVRIHHGDA LEVLRRIPDG ALAFVYLLHP DPWPKARHAK
160 170 180 190 200
RRMMNDGPLD LIAAKLKPGG EFRFGTDHPI YLRHALMVMR RHRHQFEWLA
210 220 230
KDARDFQVRP GGWPETRYEA KARAQGHEVW YFRWRRR
Length:237
Mass (Da):26,942
Last modified:May 29, 2007 - v2
Checksum:iEA69A60E7C4A03B4
GO

Sequence cautioni

The sequence ABF54843.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000356 Genomic DNA. Translation: ABF54843.1. Different initiation.
RefSeqiYP_618176.1. NC_008048.1.

Genome annotation databases

EnsemblBacteriaiABF54843; ABF54843; Sala_3140.
GeneIDi4082396.
KEGGisal:Sala_3140.
PATRICi23695658. VBISphAla23391_3234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000356 Genomic DNA. Translation: ABF54843.1. Different initiation.
RefSeqiYP_618176.1. NC_008048.1.

3D structure databases

ProteinModelPortaliQ1GNC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi317655.Sala_3140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF54843; ABF54843; Sala_3140.
GeneIDi4082396.
KEGGisal:Sala_3140.
PATRICi23695658. VBISphAla23391_3234.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciSALA317655:GHHY-3182-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13593 / LMG 18877 / RB2256.

Entry informationi

Entry nameiTRMB_SPHAL
AccessioniPrimary (citable) accession number: Q1GNC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: January 7, 2015
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.