Q1GKV6 (Q1GKV6_SILST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	TM1040_3743

Encoded on

Plasmid megaplasmid TM1040

Organism

Silicibacter sp. (strain TM1040) [Complete proteome] [HAMAP]

Taxonomic identifier

292414 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Ruegeria

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 EMBL ABF62710.1 Transferase
Technical term	Complete proteome Plasmid EMBL ABF62710.1
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	90 – 100	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	11	1	By similarity HAMAP MF_00061
	Active site	127	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	Q1GKV6 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 926C60F8C0787265	FASTA	274	28,602
	10 20 30 40 50 60 MTITEVFAPA KVNLSLHVTG RRADGYHLLD SLVAFASIGD RLRLSPAEEL SLSVSGLFAD 70 80 90 100 110 120 GVPEDSRNLV WRAAEGADWR GHIGLEKSLP HGAGIGGGSS DAGAVLNAFR SQGHTVPKAL 130 140 150 160 170 180 ALSLGADVPV CMHASAARMQ GIGEEITPIP LPKLPALLVN PGVEVPTGAV FSGLASREND 190 200 210 220 230 240 PMPSEVPRFD GVADCAGWLG AQRNDLEPAA IAAAPLIERV LSDLRASRAA LLARMSGSGS 250 260 270 TCFAIYPTLK AAHFAAYEIG AEHPEWWCVA TELS

References

[1]

"Complete sequence of megaplasmid of Silicibacter sp. TM1040." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TM1040.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000376 Genomic DNA. Translation: ABF62710.1.
RefSeq	YP_611972.1. NC_008043.1.
3D structure databases
ProteinModelPortal	Q1GKV6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1GKV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4075450.
GenomeReviews	Gene locus TM1040_3743 in contig CP000376_GR.
KEGG	sit:TM1040_3743.
NMPDR	fig|292414.1.peg.2074.
PATRIC	23383990. VBIRueSp69653_0839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	GPFAKGL.
PhylomeDB	Q1GKV6.
Enzyme and pathway databases
BioCyc	SSP292414:TM1040_3743-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q1GKV6_SILST

Accession

Primary (citable) accession number: Q1GKV6

Entry history

Integrated into UniProtKB/TrEMBL:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)