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Q1GKN8 (SYL_RUEST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TM1040_0045
OrganismRuegeria sp. (strain TM1040) (Silicibacter sp.) [Complete proteome] [HAMAP]
Taxonomic identifier292414 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria

Protein attributes

Sequence length857 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 857857Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009433

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif628 – 6325"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GKN8 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 67FE4D9302BC6DA8

FASTA85795,857
        10         20         30         40         50         60 
MSRYEASDIE ARWQKAWEEA AIFQAKEDHS KPKYYVLEMF PYPSGKLHMG HVRNYTMGDV 

        70         80         90        100        110        120 
IARYKLSTGH NVLHPMGFDA FGMPAENAAM ASGGHPKDWT YSNIDTMVEQ MKPLGLSLDW 

       130        140        150        160        170        180 
SRMFATCDPE YYGQQQALFL DFLEKGLVYR KNAVVNWDPV DMTVLANEQV ENGRGWRSGA 

       190        200        210        220        230        240 
LVERRELTQW FFKISDYSDE LLEALDGLEN WPAKVRLMQE NWIGKSRGLQ FSFERTDGEE 

       250        260        270        280        290        300 
AIEVYTTRPD TLMGASFVGI SPDHPIAKKL EAESQEVADF VAECRKGGTT EEAIETAEKL 

       310        320        330        340        350        360 
GYDTGLRVKH PLDPNWELPV WIANFILMDY GTGAIFACPA HDQRDFEFAT KYNLPIIPVL 

       370        380        390        400        410        420 
EPVEGAGELT EAYVPTKAEK VRYVRGFAGD ELQTGEEGVN TAVDKAEAEG WGQGVTKFRL 

       430        440        450        460        470        480 
RDWGLSRQRY WGCPIPVVHC PDCGVVPEKK ENLPIALPYD EDGKAIDFSV PGNPLDRHPS 

       490        500        510        520        530        540 
WRNCACPNCG KPAQRETDTM DTFVDSSWYF ARFTAPRAET PTDLDAANYW MNVDQYIGGI 

       550        560        570        580        590        600 
EHAILHLLYS RFFARAMQIC GHLPESAKEP FDALFTQGMV THAIYENAER KDDNGRAIYH 

       610        620        630        640        650        660 
YPSEVEERDG GVFVKETGER IKVIPSAKMS KSKNNVVDPL AIISTYGADT ARWFVLSDSP 

       670        680        690        700        710        720 
PERDVEWTAS GAEAAYKHLN RVWNLCDRIG EMDPDAKGEG DEELLREMHK AIRDVTLGVE 

       730        740        750        760        770        780 
SFGFNAAIAK LYGFTNTLSK SKASAAVQKQ AIKTLAQLMS PMTPHLAEDI WAHQGGEGLI 

       790        800        810        820        830        840 
ANAAWPVADE SMLVEDSVTL PIQVNGKRRG EITVAKDLDK AEVEKLALAH EAVQRVLEGA 

       850 
APKKVIVVPG RIVNVVA 

« Hide

References

[1]"Complete sequence of chromosome of Silicibacter sp. TM1040."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F., Richardson P.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TM1040.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000377 Genomic DNA. Translation: ABF62778.1.
RefSeqYP_612040.1. NC_008044.1.

3D structure databases

ProteinModelPortalQ1GKN8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292414.TM1040_0045.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF62778; ABF62778; TM1040_0045.
GeneID4078708.
KEGGsit:TM1040_0045.
PATRIC23384129. VBIRueSp69653_0907.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRSP292414:GHCT-45-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RUEST
AccessionPrimary (citable) accession number: Q1GKN8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries