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Q1GK88 (TRMB_RUEST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TM1040_0195
OrganismRuegeria sp. (strain TM1040) (Silicibacter sp.) [Complete proteome] [HAMAP]
Taxonomic identifier292414 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288229

Regions

Region233 – 2364Substrate binding By similarity

Sites

Active site1601 By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1601S-adenosyl-L-methionine By similarity
Binding site1641Substrate By similarity
Binding site1961Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GK88 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 0BAB1F955F1AFCDF

FASTA25528,721
        10         20         30         40         50         60 
MSDSDASRPS AIASDGPDAA GKHASGAPWR NFYGRFKGKT LKPNQVRDLD EMLPELSPGA 

        70         80         90        100        110        120 
VGWDENPERE PLDLEAQFQG RDVWLEVGFG GGEHLVHQAE SNPDVGIIGA EPYVNGVAML 

       130        140        150        160        170        180 
LGKLRKSSAE NVRVHAGDAR DLMDVLPAQS LSRAFLLYPD PWPKARHHRR RFVTQEHLEP 

       190        200        210        220        230        240 
LARVLKPGAI FRVATDIPDY VRQTLEEVPK AGFKWLAEGP EDWRKPWGDW ISTRYEQKAL 

       250 
REGRTPHYLT FQRQD 

« Hide

References

[1]"Complete sequence of chromosome of Silicibacter sp. TM1040."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F., Richardson P.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TM1040.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000377 Genomic DNA. Translation: ABF62928.1.
RefSeqYP_612190.1. NC_008044.1.

3D structure databases

ProteinModelPortalQ1GK88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292414.TM1040_0195.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF62928; ABF62928; TM1040_0195.
GeneID4078427.
KEGGsit:TM1040_0195.
PATRIC23384443. VBIRueSp69653_1057.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycRSP292414:GHCT-203-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RUEST
AccessionPrimary (citable) accession number: Q1GK88
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways