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Protein

Thymidylate synthase

Gene

thyA

Organism
Ruegeria sp. (strain TM1040) (Silicibacter sp.)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei159NucleophileUniRule annotation1
Binding sitei1825,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei190dUMPUniRule annotation1
Binding sitei2765,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 140dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi179 – 182dUMPUniRule annotation4
Nucleotide bindingi220 – 222dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:TM1040_1729
OrganismiRuegeria sp. (strain TM1040) (Silicibacter sp.)
Taxonomic identifieri292414 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000000636 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006821 – 277Thymidylate synthaseAdd BLAST277

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi292414.TM1040_1729.

Structurei

3D structure databases

ProteinModelPortaliQ1GFV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiIVYELLW.
OrthoDBiPOG091H02L6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.

Sequencei

Sequence statusi: Complete.

Q1GFV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQYHDALQH ILDHGVETTD RTGTGTLSCF GMQQRYDLAE GFPLVTTKKL
60 70 80 90 100
HLRSIIHELL WFLSGDTNIR YLKENGVSIW DEWADENGDL GPVYGYQWRK
110 120 130 140 150
FPRLELAEGT LGDEPLYRAG TVDQISELLD MIRKSPDSRR LIVTAWNPGD
160 170 180 190 200
VPDMALPPCH SLWQLRILGG KMHLQLYQRS ADMFLGVPFN IASYALLLHM
210 220 230 240 250
LAHVTGYEVG SFVHTMGDAH IYSNHMEQVK LQLSRSPKSL PQLRIARDVS
260 270
SIFDFKYEDF EILGYDPDPV IKAPVAV
Length:277
Mass (Da):31,557
Last modified:June 27, 2006 - v1
Checksum:i77BCABF9611CC6D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000377 Genomic DNA. Translation: ABF64462.1.
RefSeqiWP_011539057.1. NC_008044.1.

Genome annotation databases

EnsemblBacteriaiABF64462; ABF64462; TM1040_1729.
KEGGisit:TM1040_1729.
PATRICi23387640. VBIRueSp69653_2635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000377 Genomic DNA. Translation: ABF64462.1.
RefSeqiWP_011539057.1. NC_008044.1.

3D structure databases

ProteinModelPortaliQ1GFV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292414.TM1040_1729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF64462; ABF64462; TM1040_1729.
KEGGisit:TM1040_1729.
PATRICi23387640. VBIRueSp69653_2635.

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiIVYELLW.
OrthoDBiPOG091H02L6.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_RUEST
AccessioniPrimary (citable) accession number: Q1GFV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.