Q1GEF8 (T23O_SILST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	TM1040_2226

Organism

Silicibacter sp. (strain TM1040) [Complete proteome] [HAMAP]

Taxonomic identifier

292414 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Ruegeria

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Tryptophan 2,3-dioxygenase		PRO_0000360136
Regions
	Region	23 – 27	5	Substrate binding By similarity
	Region	48 – 52	5	Substrate binding By similarity
Sites
	Metal binding	237	1	Iron (heme axial ligand) By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	Heme By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GEF8 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: C97FBE2C5711FEA3	FASTA	279	32,455
	10 20 30 40 50 60 MSDSAYNPGQ DGAKMGFADA MSYGDYLHLD ALLDQQHCKS DAHDEMLFII QHQTSELWMK 70 80 90 100 110 120 LALHELQAAR EALQQGQTAE MFKMLARVSR IFEQLNSAWD VLRTMTPADY TRFREALGPS 130 140 150 160 170 180 SGFQSYQYRL IEYVLGNRNP NMLRPHEHVP EVHALLSAEL ARPSFYDEVN RYLFQTLDGH 190 200 210 220 230 240 TENLPAPRLD APHALDETIQ ERWLKVYGDI DTYWTLYELA EKLVDLEDYF RRWRFNHVTT 250 260 270 VERVIGFKRG TGGTSGVQYL RRMLSVELFP ELWTLRGDL

References

[1]

"Complete sequence of chromosome of Silicibacter sp. TM1040." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TM1040.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000377 Genomic DNA. Translation: ABF64958.1.
RefSeq	YP_614220.1. NC_008044.1.
3D structure databases
ProteinModelPortal	Q1GEF8.
SMR	Q1GEF8. Positions 20-279.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1GEF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4078217.
GenomeReviews	Gene locus TM1040_2226 in contig CP000377_GR.
KEGG	sit:TM1040_2226.
NMPDR	fig|292414.1.peg.591.
PATRIC	23388708. VBIRueSp69653_3160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	KLLVDQV.
ProtClustDB	CLSK751681.
Enzyme and pathway databases
BioCyc	SSP292414:TM1040_2226-MONOMER.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_SILST

Accession

Primary (citable) accession number: Q1GEF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program