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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Ruegeria sp. (strain TM1040) (Silicibacter sp.)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 628ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSP292414:GHCT-2687-MONOMER.
UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:TM1040_2642
OrganismiRuegeria sp. (strain TM1040) (Silicibacter sp.)
Taxonomic identifieri292414 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000000636 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Tetraacyldisaccharide 4'-kinasePRO_0000291250Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi292414.TM1040_2642.

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiHYAFERN.
OrthoDBiPOG091H000L.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1GD92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPEFWNKP PSHFDLRACL LSPLGALYAK GTATRLRKGA PTRAPVPVIC
60 70 80 90 100
VGNLNAGGTG KTPTVIWLME QLGAAGHEVH VVSRGYGGRL EGPVQVDPRR
110 120 130 140 150
HSAADVGDEP LLMAAFGEVW VARDRGAGVT EAAAAGASVI VMDDGFQNPS
160 170 180 190 200
VAKDFSIIVV DAKRGFGNGR CLPAGPLREP VATGLSRADL VLSLGDARAQ
210 220 230 240 250
ETFQSIWGAE IPVPHLTGHV EPLPTGMPWQ GTKVLAFAGI GHPEKFFATL
260 270 280 290 300
RGLGAELCRA EALEDHQTLA PALLTRLEQE ARLLGAQLVT TEKDAVRLPL
310 320 330
SFRPKVITLP VRLRVAQGDT LLARLKDIAP PPD
Length:333
Mass (Da):35,390
Last modified:June 27, 2006 - v1
Checksum:i9801FE6E77B7FDAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000377 Genomic DNA. Translation: ABF65374.1.
RefSeqiWP_011539956.1. NC_008044.1.

Genome annotation databases

EnsemblBacteriaiABF65374; ABF65374; TM1040_2642.
KEGGisit:TM1040_2642.
PATRICi23389562. VBIRueSp69653_3585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000377 Genomic DNA. Translation: ABF65374.1.
RefSeqiWP_011539956.1. NC_008044.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292414.TM1040_2642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF65374; ABF65374; TM1040_2642.
KEGGisit:TM1040_2642.
PATRICi23389562. VBIRueSp69653_3585.

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiHYAFERN.
OrthoDBiPOG091H000L.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.
BioCyciRSP292414:GHCT-2687-MONOMER.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXK_RUEST
AccessioniPrimary (citable) accession number: Q1GD92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: June 27, 2006
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.