Q1GBI7 (Q1GBI7_LACDA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171 EMBL CAI97262.1
Ordered Locus Names:	Ldb0427

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]

Taxonomic identifier

390333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus delbrueckii › Lactobacillus delbrueckii subsp. bulgaricus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792 Lyase EMBL CAI97262.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	55	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	114	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	Q1GBI7 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 514EAC9916EF6A39	FASTA	263	30,287
	10 20 30 40 50 60 MMTSRYKLTL SYDGHDFHGF QSQPGQRTIQ GTVEEILKQM TKGQEVRVFG SGRTDAGVHS 70 80 90 100 110 120 VGQVIHFDYP GREIPAANMI KALNSQLPMD MVFTDCEIVD KDFHSRFSVK GKWYRYRVSL 130 140 150 160 170 180 DGFVNPFKRF YTGHYPYPVD VEKMQEAAKD LLGRHDFTSF AASGGQIEDK VRDMYYVNVA 190 200 210 220 230 240 LDEENNEVIM DFIATGFLYN MVRILVATLL EIGNGRRPVH DLKRVIAAKN RLEVQQTAQA 250 260 CGLYLYHVFY EEVPRKYRLD LDL

References

[1]

"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution." van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. , Weissenbach J., Ehrlich S.D., Maguin E. Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954253 Genomic DNA. Translation: CAI97262.1.
RefSeq	YP_618553.1. NC_008054.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	390333.Ldb0427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI97262; CAI97262; Ldb0427.
GeneID	4083856.
KEGG	ldb:Ldb0427.
PATRIC	22216464. VBILacDel123523_0384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248673.
KO	K06173.
OMA	TPFRNVH.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	LDEL390333:GIXG-423-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q1GBI7_LACDA

Accession

Primary (citable) accession number: Q1GBI7

Entry history

Integrated into UniProtKB/TrEMBL:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)