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Q1GAS8

- SYI_LACDA

UniProt

Q1GAS8 - SYI_LACDA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
    Binding sitei600 – 6001ATPUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi913 – 9131ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLDEL390333:GIXG-744-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Ldb0748
    OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081)
    Taxonomic identifieri390333 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001259: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000022083Add
    BLAST

    Proteomic databases

    PRIDEiQ1GAS8.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi390333.Ldb0748.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1GAS8.
    SMRiQ1GAS8. Positions 1-919.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi597 – 6015"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1GAS8-1 [UniParc]FASTAAdd to Basket

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    MRVKDTLNLG KTKFPMRGNL PKREAEWEKN WEDQKFYERR LKLNEGHERF    50
    DLHDGPPFAN GNIHMGHALN KITKDIIVRS KNMEGYYAPY VPGWDTHGLP 100
    IEQQLTKQGV DRKTMDRAAY RELCRKFAME QVEKQRTDFK RLGVMGDWDH 150
    PYITLLPEFE AAEIRVFGKM YENGYIYQGK KPVYWSWSSE STLAEAEVEY 200
    HDVESPSIYI SFPVKDGKGK LSEENTYFLI WTTTPWTIPS NQGIAVNPKF 250
    DYSVVEVGDR RYVVGTDRLS AVAEILGWDS YKTVQHLKGT DMEYMVAKHP 300
    YIEGRDSLLM EAVYVTDDDG TGLVHTASGF GEDDYNTAMR YGFDVLSPMD 350
    NKGCFTEEIP DPDLVGKFYT DTNEIVKDKL SAAGNLLHYS TFVHSAAHDW 400
    RTKKPVVYRA TTQWFASISK FRDQILDQIE KTTFYPAWGK TRLYNMIKDR 450
    GDWVISRQRA WGVPLPIFYA EDGTAIVTHE TIEHVADLFA KEGSNAWFTH 500
    PVEELLPEGF TSEHSPNGKF TKETDILDVW FDSGSSWSGV QALGRAVHYP 550
    TSMYLEGSDQ YRGWFNSSLI TSVATNGVAP YKSVLSQGFT LDGQGRKMSK 600
    SLGNTIAPND VIKQMGAEII RLWVASVDAS GDVGVSMDIL RQVSEGYRKI 650
    RNTFRYMLAN TADFDPEKDR VAYKDLCKID QYLEVKLNDL VAESIVNYDK 700
    YDFADVYKLV FKFITNDLSA FYLDFAKDVL YIEGKDSHAR RSMQTVIYDA 750
    AVKLAKILAP ILPHTMGEVW GYLKEKEEDV YLSNFPEIED YADADDLKES 800
    WGEFMKLRDD VLKALEEARD QKLIGKSFEA SVTVYPGEAA KAALDKLAGE 850
    DFREILIVSN LVMGQGEVPA EAKQFDQASV LVRRAEGEVC PRCRMYRTDL 900
    GADSRLPQLC GRCASIVAGD HPEILEEGLE D 931
    Length:931
    Mass (Da):105,951
    Last modified:June 27, 2006 - v1
    Checksum:iC46C1272511A2B82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954253 Genomic DNA. Translation: CAI97575.1.
    RefSeqiYP_618812.1. NC_008054.1.

    Genome annotation databases

    EnsemblBacteriaiCAI97575; CAI97575; Ldb0748.
    GeneIDi4085353.
    KEGGildb:Ldb0748.
    PATRICi22217103. VBILacDel123523_0695.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954253 Genomic DNA. Translation: CAI97575.1 .
    RefSeqi YP_618812.1. NC_008054.1.

    3D structure databases

    ProteinModelPortali Q1GAS8.
    SMRi Q1GAS8. Positions 1-919.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390333.Ldb0748.

    Proteomic databases

    PRIDEi Q1GAS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI97575 ; CAI97575 ; Ldb0748 .
    GeneIDi 4085353.
    KEGGi ldb:Ldb0748.
    PATRICi 22217103. VBILacDel123523_0695.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LDEL390333:GIXG-744-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 11842 / DSM 20081.

    Entry informationi

    Entry nameiSYI_LACDA
    AccessioniPrimary (citable) accession number: Q1GAS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3