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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei556Aminoacyl-adenylateUniRule annotation1
Binding sitei600ATPUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1
Metal bindingi913ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLDEL390333:G1GJD-672-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ldb0748
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Taxonomic identifieri390333 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001259 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220831 – 931Isoleucine--tRNA ligaseAdd BLAST931

Proteomic databases

PRIDEiQ1GAS8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi390333.Ldb0748

Structurei

3D structure databases

ProteinModelPortaliQ1GAS8
SMRiQ1GAS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi597 – 601"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q1GAS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKDTLNLG KTKFPMRGNL PKREAEWEKN WEDQKFYERR LKLNEGHERF
60 70 80 90 100
DLHDGPPFAN GNIHMGHALN KITKDIIVRS KNMEGYYAPY VPGWDTHGLP
110 120 130 140 150
IEQQLTKQGV DRKTMDRAAY RELCRKFAME QVEKQRTDFK RLGVMGDWDH
160 170 180 190 200
PYITLLPEFE AAEIRVFGKM YENGYIYQGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
HDVESPSIYI SFPVKDGKGK LSEENTYFLI WTTTPWTIPS NQGIAVNPKF
260 270 280 290 300
DYSVVEVGDR RYVVGTDRLS AVAEILGWDS YKTVQHLKGT DMEYMVAKHP
310 320 330 340 350
YIEGRDSLLM EAVYVTDDDG TGLVHTASGF GEDDYNTAMR YGFDVLSPMD
360 370 380 390 400
NKGCFTEEIP DPDLVGKFYT DTNEIVKDKL SAAGNLLHYS TFVHSAAHDW
410 420 430 440 450
RTKKPVVYRA TTQWFASISK FRDQILDQIE KTTFYPAWGK TRLYNMIKDR
460 470 480 490 500
GDWVISRQRA WGVPLPIFYA EDGTAIVTHE TIEHVADLFA KEGSNAWFTH
510 520 530 540 550
PVEELLPEGF TSEHSPNGKF TKETDILDVW FDSGSSWSGV QALGRAVHYP
560 570 580 590 600
TSMYLEGSDQ YRGWFNSSLI TSVATNGVAP YKSVLSQGFT LDGQGRKMSK
610 620 630 640 650
SLGNTIAPND VIKQMGAEII RLWVASVDAS GDVGVSMDIL RQVSEGYRKI
660 670 680 690 700
RNTFRYMLAN TADFDPEKDR VAYKDLCKID QYLEVKLNDL VAESIVNYDK
710 720 730 740 750
YDFADVYKLV FKFITNDLSA FYLDFAKDVL YIEGKDSHAR RSMQTVIYDA
760 770 780 790 800
AVKLAKILAP ILPHTMGEVW GYLKEKEEDV YLSNFPEIED YADADDLKES
810 820 830 840 850
WGEFMKLRDD VLKALEEARD QKLIGKSFEA SVTVYPGEAA KAALDKLAGE
860 870 880 890 900
DFREILIVSN LVMGQGEVPA EAKQFDQASV LVRRAEGEVC PRCRMYRTDL
910 920 930
GADSRLPQLC GRCASIVAGD HPEILEEGLE D
Length:931
Mass (Da):105,951
Last modified:June 27, 2006 - v1
Checksum:iC46C1272511A2B82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954253 Genomic DNA Translation: CAI97575.1
RefSeqiWP_011543780.1, NZ_JQAV01000001.1

Genome annotation databases

EnsemblBacteriaiCAI97575; CAI97575; Ldb0748
GeneIDi4085353
KEGGildb:Ldb0748
PATRICifig|390333.13.peg.51

Similar proteinsi

Entry informationi

Entry nameiSYI_LACDA
AccessioniPrimary (citable) accession number: Q1GAS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health