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Q1GAP2 (TYSY_LACDA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Ldb0787
OrganismLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]
Taxonomic identifier390333 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000000614

Sites

Active site2001 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GAP2 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: D0EC7D5781914CFE

FASTA31836,371
        10         20         30         40         50         60 
MANQDQAYLD LLKKIMTEGN DKNDRTGTGT RSLFGAQMRF DLSQGFPILT TKRVPFGLIK 

        70         80         90        100        110        120 
SELLWFLRGD TNIRFLLEHK NHIWDEWAFK NWVTSPEYQG PDMTDFGLRS QKDPGFKAVY 

       130        140        150        160        170        180 
DEEMQKFCQR ILDDEAFAQK YGNLGDVYGA QWRHWGKRDG GFIDQIADVI EQIKTNPDSR 

       190        200        210        220        230        240 
RLIVTAWNPE DVPSSALPPC HVLFQFYVAD GKLSLQLYQR SGDMFLGVPF NIASYSLLLS 

       250        260        270        280        290        300 
LIARETGLEV GEFVHTIGDA HIYKNHFAQV EEQLKRKPFD APTLWLNPGK KKVADFEMAD 

       310 
IKLVNYQHGS TIKAPVAV 

« Hide

References

[1]"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution."
van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. expand/collapse author list , Weissenbach J., Ehrlich S.D., Maguin E.
Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR954253 Genomic DNA. Translation: CAI97614.1.
RefSeqYP_618848.1. NC_008054.1.

3D structure databases

ProteinModelPortalQ1GAP2.
SMRQ1GAP2. Positions 5-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390333.Ldb0787.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI97614; CAI97614; Ldb0787.
GeneID4083847.
KEGGldb:Ldb0787.
PATRIC22217181. VBILacDel123523_0734.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMADVHLYSN.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycLDEL390333:GIXG-783-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 2 hits.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_LACDA
AccessionPrimary (citable) accession number: Q1GAP2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways