Reviewed, UniProtKB/Swiss-Prot Q1G9Z4 (BGAL_LACDA)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	Ldb1201

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]

Taxonomic identifier

390333 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	1008 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1008	1007	Beta-galactosidase		PRO_0000250751
Sites
	Active site	465	1	Proton donor By similarity
	Active site	532	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1G9Z4-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4681BFCA448F731F	FASTA	1,008	114,160
	10 20 30 40 50 60 MSNKLVKEKR VDQADLAWLT DPEVYEVNTI PPHSDHESFQ SQEELEEGKS SLVQSLDGDW 70 80 90 100 110 120 LIDYAENGQG PVNFYAEDFD DSNFKSVKVP GNLELQGFGQ PQYVNVQYPW DGSEEIFPPQ 130 140 150 160 170 180 IPSKNPLASY VRYFDLDEAF WDKEVSLKFD GAATAIYVWL NGHFVGYGED SFTPSEFMVT 190 200 210 220 230 240 KFLKKENNRL AVALYKYSSA SWLEDQDFWR MSGLFRSVTL QAKPRLHLED LKLTASLTDN 250 260 270 280 290 300 YQKGKLEVEA NIAYRLPNAS FKLEVRDSEG DLVAEKLGPI RSEQLEFTLA DLPVAAWSAE 310 320 330 340 350 360 KPNLYQVRLY LYQAGSLLEV SRQEVGFRNF ELKDGIMYLN GQRIVFKGAN RHEFDSKLGR 370 380 390 400 410 420 AITEEDMIWD IKTMKRSNIN AVRCSHYPNQ SLFYRLCDKY GLYVIDEANL ESHGTWEKVG 430 440 450 460 470 480 GHEDPSFNVP GDDQHWLGAS LSRVKNMMAR DKNHASILIW SLGNESYAGT VFAQMADYVR 490 500 510 520 530 540 KADPTRVQHY EGVTHNRKFD DATQIESRMY APAKVIEEYL TNKPAKPFIS VEYAHAMGNS 550 560 570 580 590 600 VGDLAAYTAL EKYPHYQGGF IWDWIDQGLE KDGHLLYGGD FDDRPTDYEF CGNGLVFADR 610 620 630 640 650 660 TESPKLANVK ALYANLKLEV KDGQLFLKND NLFTNSSSYY FLTSLLVDGK LTYQSRPLTF 670 680 690 700 710 720 GLEPGESGTF ALPWPEVADE KGEVVYRVTA HLKEDLPWAD EGFTVAEAEE VAQKLPEFKP 730 740 750 760 770 780 EGRPDLVDSD YNLGLKGNNF QILFSKVKGW PVSLKYAGRE YLKRLPEFTF WRALTDNDRG 790 800 810 820 830 840 AGYGYDLARW ENAGKYARLK DISCEVKEDS VLVKTAFTLP VALKGDLTVT YEVDGRGKIA 850 860 870 880 890 900 VTADFPGAEE AGLLPAFGLN LALPKELTDY RYYGLGPNES YPDRLEGNYL GIYQGAVKKN 910 920 930 940 950 960 FSPYLRPQET GNRSKVRWYQ LFDEKGGLEF TANGADLNLS ALPYSAAQIE AADHAFELTN 970 980 990 1000 NYTWVRALSA QMGVGGDDSW GQKVHPEFCL DAQKARQLRL VIQPLLLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved." Leong-Morgenthaler P.M., Zwahlen M.-C., Hottinger H. J. Bacteriol. 173:1951-1957(1991) [PubMed: 1705929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution." van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. , Weissenbach J., Ehrlich S.D., Maguin E. Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed: 16754859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55068 Genomic DNA. Translation: AAA25244.1. CR954253 Genomic DNA. Translation: CAI98003.1.
RefSeq	YP_619096.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1G9Z4.
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Genome annotation databases
GeneID	4085367.
GenomeReviews	Gene locus Ldb1201 in contig CR954253_GR.
KEGG	ldb:Ldb1201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1G9Z4.
OMA	DEYGLYL.
Enzyme and pathway databases
BioCyc	LDEL390333:LDB1201-MON.
Family and domain databases
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LACDA

Accession

Primary (citable) accession number: Q1G9Z4
Secondary accession number(s): P20043

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)