Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q1G971 (SYL_LACDA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ldb1570
OrganismLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]
Taxonomic identifier390333 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334766

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif573 – 5775"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1G971 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 605222ED8C41D8B7

FASTA80491,961
        10         20         30         40         50         60 
MYNHKVVEKK WQKYWLENKT FKTGTDPEKP KYYVLDMFPY PSGKGLHVGH PEGYTATDIM 

        70         80         90        100        110        120 
ARMKRAQGYN VLHPMGWDAF GLPAEQYALQ TGNDPATFTD ENIAHFKKQL QALGFSYDWD 

       130        140        150        160        170        180 
REIKTTDPNY YKWTQWIFEQ MYKMGLAYEA EVPVNWSPDL GTVVANEEVI DGKTERGGYP 

       190        200        210        220        230        240 
VYRRKMRQWM LKITAYADRL LDDLDDLDWP EPIKEMQRNW IGRSVGAQVT FKIKDSDKSF 

       250        260        270        280        290        300 
AVFTTRPDTL FGCSYTVLAP ENELVKEITS PEQKEAVDAY IKSIESKSDL ERTDLNKDKT 

       310        320        330        340        350        360 
GVFTGAYAIN PVNGEEVPVW ISDYVLATYG TGAVMAVPAH DERDYAFATK FDLPIKEVVE 

       370        380        390        400        410        420 
GGDISKEAFA GDGVHVNSDF LNGLHNEEAK AKMVDWLTEK GVGEKKVNYK MRDWNFSRQR 

       430        440        450        460        470        480 
YWGEPIPVIH WEDGETTLVP EDELPLRLPK ESNIKPSGTP ESPLANLTDW VNVVDENGRK 

       490        500        510        520        530        540 
GKRETNTMPQ WAGSSWYFLR YIDPHNDKAL ADPELLKKWM PVDLYIGGAE HATLHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVPTKEPFQK LYNQGLILKN HEKMSKSRGN VVNPDDVVDE YGADSLRTYE 

       610        620        630        640        650        660 
MFMGPLNASI DWDDNGPSGV KKFLDRVWRT FVNDLDLDPI PSEKITDKND GKLDKIYNET 

       670        680        690        700        710        720 
VKTVTEHFEE LRFNTAISQM MVFMNACQKV DKIPREYAEG FVKLMAPVAP HMMEEIWHVF 

       730        740        750        760        770        780 
GHDESVQFAA WPTYDASKLV ESTVEMAVTV NGKKRGNFQI AKDASREEAQ AAATALTHVK 

       790        800 
EFLEGKEIKK VIVVPNKIVN IVAK 

« Hide

References

[1]"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution."
van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. expand/collapse author list , Weissenbach J., Ehrlich S.D., Maguin E.
Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR954253 Genomic DNA. Translation: CAI98369.1.
RefSeqYP_619369.1. NC_008054.1.

3D structure databases

ProteinModelPortalQ1G971.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390333.Ldb1570.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI98369; CAI98369; Ldb1570.
GeneID4085372.
KEGGldb:Ldb1570.
PATRIC22218594. VBILacDel123523_1413.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLDEL390333:GIXG-1550-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACDA
AccessionPrimary (citable) accession number: Q1G971
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries