Q1G8Z0 (SYC_LACDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Ldb1680

Organism

Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]

Taxonomic identifier

390333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus delbrueckii › Lactobacillus delbrueckii subsp. bulgaricus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000332838
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	271 – 275	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1G8Z0 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 5EC6439AEE65F809	FASTA	474	53,673
	10 20 30 40 50 60 MKLFNTLTRQ KEEFKPLVPG QVSMYVCGPT VYNYIHIGNA RSAIAFDTIR RYFEYKGYKV 70 80 90 100 110 120 NYVSNFTDVD DKMINEAGAE GTTVPELAER YIQAFLEDTR ALNIEEATLH PRATHEIPAI 130 140 150 160 170 180 IDFIQTLIDK GYAYEADGDV YYRTKKFADY GHLSDQNIDQ LEEGASQHVN DEEQGRKEDP 190 200 210 220 230 240 IDFALWKGQK AADEIAWDSP WGKGRPGWHI ECSVMSTKYL GDTLDIHGGG QDLEFPHHEN 250 260 270 280 290 300 EIAQSEAKTG KKFVNYWLHN GFVTVGKDEE KMSKSLHNCV TVHDILKNVD PQVLRFFMAS 310 320 330 340 350 360 VQYRSQINYS EENLEQAANI LGRFKNTLEG INYRLADATE GLPDPDLAKL VTETTAKFEA 370 380 390 400 410 420 AMDDDFNVQN ALTAIYEALP AVNSNANAEK ADKESLRLFA KKLAAWLSVF GLDVDKLLAK 430 440 450 460 470 EAGDDDAVIE ELVAQRTEAR KNKDWAKSDE LRDQLKEMGV VLKDTPQGTR WSRE

References

[1]

"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution." van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. , Weissenbach J., Ehrlich S.D., Maguin E. Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954253 Genomic DNA. Translation: CAI98469.1.
RefSeq	YP_619450.1. NC_008054.1.
3D structure databases
ProteinModelPortal	Q1G8Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	390333.Ldb1680.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI98469; CAI98469; Ldb1680.
GeneID	4084553.
KEGG	ldb:Ldb1680.
PATRIC	22218804. VBILacDel123523_1508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	LDEL390333:GIXG-1659-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_LACDA

Accession

Primary (citable) accession number: Q1G8Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program