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Protein

Dihydroorotate dehydrogenase A (fumarate)

Gene

pyrD

Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor.By similarity

Catalytic activityi

(S)-dihydroorotate + fumarate = orotate + succinate.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211FMNBy similarity
Binding sitei46 – 461SubstrateBy similarity
Binding sitei130 – 1301FMNBy similarity
Binding sitei130 – 1301SubstrateBy similarity
Active sitei133 – 1331Nucleophile
Binding sitei168 – 1681FMNBy similarity
Binding sitei194 – 1941FMN; via carbonyl oxygenBy similarity
Binding sitei220 – 2201FMN; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 472FMNBy similarity
Nucleotide bindingi246 – 2472FMNBy similarity
Nucleotide bindingi268 – 2692FMNBy similarity

GO - Molecular functioni

  1. dihydroorotate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciLDEL390333:GIXG-2083-MONOMER.
UniPathwayiUPA00070.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase A (fumarate) (EC:1.3.98.1)
Short name:
DHOD A
Short name:
DHODase A
Short name:
DHOdehase A
Gene namesi
Name:pyrD
Ordered Locus Names:Ldb2114
OrganismiLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Taxonomic identifieri390333 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001259: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Dihydroorotate dehydrogenase A (fumarate)PRO_1000024135Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi390333.Ldb2114.

Structurei

3D structure databases

ProteinModelPortaliQ1G864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 745Substrate bindingBy similarity
Regioni195 – 1962Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0167.
HOGENOMiHOG000225105.
KOiK00226.
OMAiETPMGML.
OrthoDBiEOG6NPM9S.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00224. DHO_dh_type1.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1G864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNTHVNLPG LDLKNPVMPA SGTFGFGDVP AAQKFDLNDL GAMVIKTTTP
60 70 80 90 100
HATTGNPQPQ IAILEDGVLN SVGLTNPGVD QVISEKLTKL RHQYLDLPIM
110 120 130 140 150
ASVGGDSEDD YVEVAKKLSA SGLVNALEIN VSCPNVAQGG MSFGVHAGVV
160 170 180 190 200
EELTKKIKMA VALPIYVKLT PNVTDIVEIA KAAESGGADG ISMINTVLGM
210 220 230 240 250
RIDVKTRKPL LGHNMGGLSG EAVKPIAIRM ISQVRQVTQL PIIGMGGIST
260 270 280 290 300
AQDVIEFILA GANAVAVGSA HFEDELAAKH IAENLPAELE KLGIEDINDL

VGQVKFN
Length:307
Mass (Da):32,181
Last modified:June 27, 2006 - v1
Checksum:i799B034239D6C885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954253 Genomic DNA. Translation: CAI98852.1.
RefSeqiWP_011544329.1. NC_008054.1.
YP_619726.1. NC_008054.1.

Genome annotation databases

EnsemblBacteriaiCAI98852; CAI98852; Ldb2114.
GeneIDi4084395.
KEGGildb:Ldb2114.
PATRICi22219615. VBILacDel123523_1862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954253 Genomic DNA. Translation: CAI98852.1.
RefSeqiWP_011544329.1. NC_008054.1.
YP_619726.1. NC_008054.1.

3D structure databases

ProteinModelPortaliQ1G864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390333.Ldb2114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI98852; CAI98852; Ldb2114.
GeneIDi4084395.
KEGGildb:Ldb2114.
PATRICi22219615. VBILacDel123523_1862.

Phylogenomic databases

eggNOGiCOG0167.
HOGENOMiHOG000225105.
KOiK00226.
OMAiETPMGML.
OrthoDBiEOG6NPM9S.

Enzyme and pathway databases

UniPathwayiUPA00070.
BioCyciLDEL390333:GIXG-2083-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00224. DHO_dh_type1.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778.

Entry informationi

Entry nameiPYRDA_LACDA
AccessioniPrimary (citable) accession number: Q1G864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.