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Q1G864 (PYRDA_LACDA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase A (fumarate)

Short name=DHOD A
Short name=DHODase A
Short name=DHOdehase A
EC=1.3.98.1
Gene names
Name:pyrD
Ordered Locus Names:Ldb2114
OrganismLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]
Taxonomic identifier390333 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor By similarity. HAMAP-Rule MF_00224

Catalytic activity

(S)-dihydroorotate + fumarate = orotate + succinate. HAMAP-Rule MF_00224

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP-Rule MF_00224

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway. HAMAP-Rule MF_00224

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00224

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00224.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCytoplasm
   LigandFlavoprotein
FMN
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   UncategorizedOBSOLETE:dihydroorotate oxidase (fumarate) activity

Inferred from electronic annotation. Source: UniProtKB-EC

   Biological_process'de novo' UMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

'de novo' pyrimidine nucleobase biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Dihydroorotate dehydrogenase A (fumarate) HAMAP-Rule MF_00224
PRO_1000024135

Regions

Nucleotide binding46 – 472FMN By similarity
Nucleotide binding246 – 2472FMN By similarity
Nucleotide binding268 – 2692FMN By similarity
Region70 – 745Substrate binding By similarity
Region195 – 1962Substrate binding By similarity

Sites

Active site1331Nucleophile
Binding site211FMN By similarity
Binding site461Substrate By similarity
Binding site1301FMN By similarity
Binding site1301Substrate By similarity
Binding site1681FMN By similarity
Binding site1941FMN; via carbonyl oxygen By similarity
Binding site2201FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1G864 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 799B034239D6C885

FASTA30732,181
        10         20         30         40         50         60 
MVNTHVNLPG LDLKNPVMPA SGTFGFGDVP AAQKFDLNDL GAMVIKTTTP HATTGNPQPQ 

        70         80         90        100        110        120 
IAILEDGVLN SVGLTNPGVD QVISEKLTKL RHQYLDLPIM ASVGGDSEDD YVEVAKKLSA 

       130        140        150        160        170        180 
SGLVNALEIN VSCPNVAQGG MSFGVHAGVV EELTKKIKMA VALPIYVKLT PNVTDIVEIA 

       190        200        210        220        230        240 
KAAESGGADG ISMINTVLGM RIDVKTRKPL LGHNMGGLSG EAVKPIAIRM ISQVRQVTQL 

       250        260        270        280        290        300 
PIIGMGGIST AQDVIEFILA GANAVAVGSA HFEDELAAKH IAENLPAELE KLGIEDINDL 


VGQVKFN 

« Hide

References

[1]"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution."
van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. expand/collapse author list , Weissenbach J., Ehrlich S.D., Maguin E.
Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR954253 Genomic DNA. Translation: CAI98852.1.
RefSeqYP_619726.1. NC_008054.1.

3D structure databases

ProteinModelPortalQ1G864.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390333.Ldb2114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI98852; CAI98852; Ldb2114.
GeneID4084395.
KEGGldb:Ldb2114.
PATRIC22219615. VBILacDel123523_1862.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0167.
HOGENOMHOG000225105.
KOK00226.
OMAVTDIVAM.
OrthoDBEOG6NPM9S.

Enzyme and pathway databases

BioCycLDEL390333:GIXG-2083-MONOMER.
UniPathwayUPA00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00224. DHO_dh_type1.
InterProIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEPS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRDA_LACDA
AccessionPrimary (citable) accession number: Q1G864
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways