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Q1G7Q5 (DDL_LACDA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Ldb0185
OrganismLactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) [Complete proteome] [HAMAP]
Taxonomic identifier390333 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341117

Regions

Domain134 – 345212ATP-grasp
Nucleotide binding170 – 22556ATP By similarity

Sites

Metal binding2981Magnesium or manganese 1 By similarity
Metal binding3121Magnesium or manganese 1 By similarity
Metal binding3121Magnesium or manganese 2 By similarity
Metal binding3141Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1G7Q5 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: EA32581F4697DA87

FASTA36240,098
        10         20         30         40         50         60 
MTKKTQVGLI FGGNSSEYEV SITSAGNIYK AIDKDKFDVH PIWITNSGYV ASEADSYKVL 

        70         80         90        100        110        120 
EEPGYMVEKN DKTANLSNLI ELASRPELDV LFPIVHGNLG EDGVLQGLFR LIDKPFVGVD 

       130        140        150        160        170        180 
VLAAAVTMDK EFTKILAQRA GVPVAKWVSI KRYEYEDAKN QKLSYDWVSG QLGTSNLFVK 

       190        200        210        220        230        240 
PSNQGSSVGI THVTDDSNYA EALAEAFKYD DKVLVEEGIV GTEVETAVLG NDHPVVSGVG 

       250        260        270        280        290        300 
QIINADNTWY SYENKYSTES TTTLQIPAQI PAETAEKVQE NALKIYQITE CSGMARVDSM 

       310        320        330        340        350        360 
LRESDGEVIF NELNALPGFT NISMYPKLFE EAGISYPDLI TRLIRLAEER YAHKKTLLRK 


HD 

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References

[1]"The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution."
van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P., Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R., Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P. expand/collapse author list , Weissenbach J., Ehrlich S.D., Maguin E.
Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11842 / DSM 20081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR954253 Genomic DNA. Translation: CAI97025.1.
RefSeqYP_618371.1. NC_008054.1.

3D structure databases

ProteinModelPortalQ1G7Q5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390333.Ldb0185.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI97025; CAI97025; Ldb0185.
GeneID4084556.
KEGGldb:Ldb0185.
PATRIC22216014. VBILacDel123523_0165.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycLDEL390333:GIXG-183-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LACDA
AccessionPrimary (citable) accession number: Q1G7Q5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 27, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways