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Protein

Myosin-IIIb

Gene

Myo3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable actin-based motor with a protein kinase activity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: MGI
  • protein serine/threonine kinase activity Source: MGI

GO - Biological processi

  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI
  • positive regulation of filopodium assembly Source: MGI
  • protein autophosphorylation Source: MGI
  • response to stimulus Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Motor protein, Myosin, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-IIIb (EC:2.7.11.1)
Gene namesi
Name:Myo3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2448580. Myo3b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • filopodium tip Source: MGI
  • myosin complex Source: UniProtKB-KW
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI
  • stereocilium tip Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002778301 – 1305Myosin-IIIbAdd BLAST1305

Proteomic databases

PaxDbiQ1EG27.
PRIDEiQ1EG27.

PTM databases

iPTMnetiQ1EG27.
PhosphoSitePlusiQ1EG27.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042064.
CleanExiMM_MYO3B.
ExpressionAtlasiQ1EG27. baseline and differential.
GenevisibleiQ1EG27. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055362.

Structurei

Secondary structure

11305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1223 – 1225Combined sources3
Helixi1234 – 1241Combined sources8
Helixi1273 – 1275Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ET0X-ray2.30B/D1252-1305[»]
5ET1X-ray1.65C/D1216-1251[»]
ProteinModelPortaliQ1EG27.
SMRiQ1EG27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 281Protein kinasePROSITE-ProRule annotationAdd BLAST267
Domaini331 – 1046Myosin motorAdd BLAST716
Domaini1048 – 1077IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini1075 – 1104IQ 2PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

In the C-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.Curated
In the N-terminal section; belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.Curated
Contains 2 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
KOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007169.
HOVERGENiHBG052555.
InParanoidiQ1EG27.
KOiK08834.
PhylomeDBiQ1EG27.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR011009. Kinase-like_dom.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1EG27-1) [UniParc]FASTAAdd to basket
Also known as: Myosin IIIB1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLGLESLPD PMETWEIIET IGKGTYGKVY KVANKRDGSL AAVKVLDPVS
60 70 80 90 100
DMDEEIEAEY NILQFLPSHP NVVKFYGMFY KADRCVGGQL WLVLELCNGG
110 120 130 140 150
SVTELVKGLL RCGKRLDEAV ISYILYGALL GLQHLHCHRI IHRDVKGNNI
160 170 180 190 200
LLTTEGGVKL VDFGVSAQLT STRLRRNTSV GTPFWMAPEV IACEQQYDSS
210 220 230 240 250
YDARCDVWSL GITAIELGDG DPPLFEMHPV KMLFKIPRNP PPTLLHPDSW
260 270 280 290 300
CEEFNHFISQ CLIKDFEKRP SVTHLLDHPF IKGTQGKVLC LQKQLAKVLQ
310 320 330 340 350
DQKHRNPVAK TRHERMHTGR PHRVEDAGKC CLEDDLVNLE VLDEDTIIYW
360 370 380 390 400
LQKRYADALI YTYVGDILIA LNPFQNLSIY SPQFSRLYHG VKRSSNPPHI
410 420 430 440 450
FASADNAYQC LVTFSKDQCI VISGESGSGK TESAHLIVQH LTFLGKADNQ
460 470 480 490 500
TLRQKILQVN SLVEAFGNAR TAINDNSSRF GKYLEMMFTP TGAVMGARIS
510 520 530 540 550
EYLLEKSRVI QQAAGEKNFH IFYYIYAGLY HQKKLAEFRL PEEKPPRYIA
560 570 580 590 600
GETERVMQDI TSKESYRTQF EAIQHCFKII GFADKEVHSV YRILAGILNI
610 620 630 640 650
GSIEFAAISS QHQTDKSEVP NPEALENAAC VLCISSEELQ EALTSHCVVT
660 670 680 690 700
RGETIVRANT VDRAEDVRDA MSKALYGRLF SWIVNRINTL LQPDKNICSA
710 720 730 740 750
EDRMNVGILD IFGFEDFQRN SFEQLCINIA NEQIQYYFNQ HVFALEQMEY
760 770 780 790 800
KNEGVDAVLV QYEDNRPLLD MFLQKPLGLL ALLDEESRFP QGTDQTLVDK
810 820 830 840 850
FEDNLRCKFF WRPKGVELCF GIQHYAGPVL YDASGVLEKN RDTLPADVVV
860 870 880 890 900
VLRTSENKLL QQLFSIPLTK TGNLAQTRAK ITASSRSLPP HFSAGRAKVD
910 920 930 940 950
TLEVIRHPEE TTNMKRQTMA SYFRYSLMDL LSKMVVGQPH FIRCIKPNDD
960 970 980 990 1000
RKALQFSQDR VLAQLRSTGI LETVSIRRQG YSHRIFFEEF VKRYYYLAFR
1010 1020 1030 1040 1050
AHQTPPANKE SCVAILEKSR LDHWVLGKTK VFLKYYHVEQ LNLLLREVMG
1060 1070 1080 1090 1100
RVVMLQAYTK GWLGARRYKR AKEKREKGAI TIQSAWRGYD ARRKLKQRSR
1110 1120 1130 1140 1150
RRSESEAHIH TVLQTTPDQK YCPDSGGESN RGHEETSRNC PAEADTDGHP
1160 1170 1180 1190 1200
QAQSPPTGCD VTSGHADTAA GYTVAELSVA GTDVSPSLVY HTASAHQRLS
1210 1220 1230 1240 1250
PCEDSLKPGS EEGLSQKQRA PRRRCQQPKM LSSPEDTMYY NQLNGTLEYQ
1260 1270 1280 1290 1300
GSQRKPRKLG QIKVLDGEDQ YYKCLSPGAC APEETHSVHP FFFSSSPRED

PFAQH
Length:1,305
Mass (Da):148,099
Last modified:December 16, 2008 - v2
Checksum:i9B8752F2704C7C80
GO
Isoform 2 (identifier: Q1EG27-2) [UniParc]FASTAAdd to basket
Also known as: Myosin IIIB2

The sequence of this isoform differs from the canonical sequence as follows:
     898-898: K → KKSPHSVPSYVLNTSPPK
     1149-1209: Missing.

Show »
Length:1,261
Mass (Da):143,762
Checksum:iDCCCFDBD868177A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti505E → K in AAX59998 (Ref. 1) Curated1
Sequence conflicti505E → K in AAX59999 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036028898K → KKSPHSVPSYVLNTSPPK in isoform 2. 1 Publication1
Alternative sequenceiVSP_0231121149 – 1209Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY830392 mRNA. Translation: AAX59998.2.
AY830393 mRNA. Translation: AAX59999.2.
AL845263, AL929164 Genomic DNA. Translation: CAM21397.1.
AL929164, AL845263 Genomic DNA. Translation: CAM17979.1.
RefSeqiNP_796350.2. NM_177376.4.
XP_017174528.1. XM_017319039.1. [Q1EG27-1]
UniGeneiMm.99648.

Genome annotation databases

EnsembliENSMUST00000112243; ENSMUSP00000107862; ENSMUSG00000042064. [Q1EG27-1]
GeneIDi329421.
KEGGimmu:329421.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY830392 mRNA. Translation: AAX59998.2.
AY830393 mRNA. Translation: AAX59999.2.
AL845263, AL929164 Genomic DNA. Translation: CAM21397.1.
AL929164, AL845263 Genomic DNA. Translation: CAM17979.1.
RefSeqiNP_796350.2. NM_177376.4.
XP_017174528.1. XM_017319039.1. [Q1EG27-1]
UniGeneiMm.99648.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ET0X-ray2.30B/D1252-1305[»]
5ET1X-ray1.65C/D1216-1251[»]
ProteinModelPortaliQ1EG27.
SMRiQ1EG27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055362.

PTM databases

iPTMnetiQ1EG27.
PhosphoSitePlusiQ1EG27.

Proteomic databases

PaxDbiQ1EG27.
PRIDEiQ1EG27.

Protocols and materials databases

DNASUi329421.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112243; ENSMUSP00000107862; ENSMUSG00000042064. [Q1EG27-1]
GeneIDi329421.
KEGGimmu:329421.

Organism-specific databases

CTDi140469.
MGIiMGI:2448580. Myo3b.

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
KOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007169.
HOVERGENiHBG052555.
InParanoidiQ1EG27.
KOiK08834.
PhylomeDBiQ1EG27.

Miscellaneous databases

PROiQ1EG27.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042064.
CleanExiMM_MYO3B.
ExpressionAtlasiQ1EG27. baseline and differential.
GenevisibleiQ1EG27. MM.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR011009. Kinase-like_dom.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO3B_MOUSE
AccessioniPrimary (citable) accession number: Q1EG27
Secondary accession number(s): A2AR19, Q1EG26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 16, 2008
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.