Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin-IIIb

Gene

Myo3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable actin-based motor with a protein kinase activity (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: MGI
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: InterPro
  • protein serine/threonine kinase activity Source: MGI

GO - Biological processi

  • cochlea morphogenesis Source: UniProtKB
  • microtubule-based movement Source: InterPro
  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI
  • positive regulation of filopodium assembly Source: MGI
  • protein autophosphorylation Source: MGI
  • response to stimulus Source: UniProtKB-KW
  • sensory perception of sound Source: UniProtKB
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionActin-binding, Kinase, Motor protein, Myosin, Serine/threonine-protein kinase, Transferase
Biological processHearing, Sensory transduction, Vision
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-IIIb (EC:2.7.11.1)
Gene namesi
Name:Myo3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2448580 Myo3b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

MYO3B single knockout mice do not exhibit early hearing impairment whereas mice with a double knockout of MYO3A and MYO3B are profoundly deaf at 1 month of age. Cochlear hair bundles have abnormally long stereocilia and show dynamic shape defects during development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002778301 – 1305Myosin-IIIbAdd BLAST1305

Proteomic databases

PaxDbiQ1EG27
PRIDEiQ1EG27

PTM databases

iPTMnetiQ1EG27
PhosphoSitePlusiQ1EG27

Expressioni

Tissue specificityi

Expressed in the cochlear hair cells (at protein level) (PubMed:26754646). Expressed in utricle hair bundles (at protein level) (PubMed:26926603).2 Publications

Gene expression databases

BgeeiENSMUSG00000042064
CleanExiMM_MYO3B
ExpressionAtlasiQ1EG27 baseline and differential
GenevisibleiQ1EG27 MM

Interactioni

Subunit structurei

Interacts (via C-terminus) with ESPN (PubMed:26785147, PubMed:26926603). Interacts (via C-terminus) with ESPNL (PubMed:26926603).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi236763, 1 interactor
STRINGi10090.ENSMUSP00000055362

Structurei

Secondary structure

11305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1223 – 1225Combined sources3
Helixi1234 – 1241Combined sources8
Helixi1273 – 1275Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ET0X-ray2.30B/D1252-1305[»]
5ET1X-ray1.65C/D1216-1251[»]
ProteinModelPortaliQ1EG27
SMRiQ1EG27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 281Protein kinasePROSITE-ProRule annotationAdd BLAST267
Domaini331 – 1046Myosin motorPROSITE-ProRule annotationAdd BLAST716
Domaini1048 – 1077IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini1075 – 1104IQ 2PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni927 – 949Actin-bindingPROSITE-ProRule annotationAdd BLAST23

Sequence similaritiesi

In the C-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.Curated
In the N-terminal section; belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0587 Eukaryota
KOG4229 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00900000140778
HOGENOMiHOG000007169
HOVERGENiHBG052555
InParanoidiQ1EG27
KOiK08834
PhylomeDBiQ1EG27

Family and domain databases

CDDicd01379 MYSc_Myo3, 1 hit
Gene3Di3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR011009 Kinase-like_dom_sf
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR036083 MYSc_Myo3
IPR027417 P-loop_NTPase
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00612 IQ, 1 hit
PF00063 Myosin_head, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00015 IQ, 2 hits
SM00242 MYSc, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 2 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1EG27-1) [UniParc]FASTAAdd to basket
Also known as: Myosin IIIB1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLGLESLPD PMETWEIIET IGKGTYGKVY KVANKRDGSL AAVKVLDPVS
60 70 80 90 100
DMDEEIEAEY NILQFLPSHP NVVKFYGMFY KADRCVGGQL WLVLELCNGG
110 120 130 140 150
SVTELVKGLL RCGKRLDEAV ISYILYGALL GLQHLHCHRI IHRDVKGNNI
160 170 180 190 200
LLTTEGGVKL VDFGVSAQLT STRLRRNTSV GTPFWMAPEV IACEQQYDSS
210 220 230 240 250
YDARCDVWSL GITAIELGDG DPPLFEMHPV KMLFKIPRNP PPTLLHPDSW
260 270 280 290 300
CEEFNHFISQ CLIKDFEKRP SVTHLLDHPF IKGTQGKVLC LQKQLAKVLQ
310 320 330 340 350
DQKHRNPVAK TRHERMHTGR PHRVEDAGKC CLEDDLVNLE VLDEDTIIYW
360 370 380 390 400
LQKRYADALI YTYVGDILIA LNPFQNLSIY SPQFSRLYHG VKRSSNPPHI
410 420 430 440 450
FASADNAYQC LVTFSKDQCI VISGESGSGK TESAHLIVQH LTFLGKADNQ
460 470 480 490 500
TLRQKILQVN SLVEAFGNAR TAINDNSSRF GKYLEMMFTP TGAVMGARIS
510 520 530 540 550
EYLLEKSRVI QQAAGEKNFH IFYYIYAGLY HQKKLAEFRL PEEKPPRYIA
560 570 580 590 600
GETERVMQDI TSKESYRTQF EAIQHCFKII GFADKEVHSV YRILAGILNI
610 620 630 640 650
GSIEFAAISS QHQTDKSEVP NPEALENAAC VLCISSEELQ EALTSHCVVT
660 670 680 690 700
RGETIVRANT VDRAEDVRDA MSKALYGRLF SWIVNRINTL LQPDKNICSA
710 720 730 740 750
EDRMNVGILD IFGFEDFQRN SFEQLCINIA NEQIQYYFNQ HVFALEQMEY
760 770 780 790 800
KNEGVDAVLV QYEDNRPLLD MFLQKPLGLL ALLDEESRFP QGTDQTLVDK
810 820 830 840 850
FEDNLRCKFF WRPKGVELCF GIQHYAGPVL YDASGVLEKN RDTLPADVVV
860 870 880 890 900
VLRTSENKLL QQLFSIPLTK TGNLAQTRAK ITASSRSLPP HFSAGRAKVD
910 920 930 940 950
TLEVIRHPEE TTNMKRQTMA SYFRYSLMDL LSKMVVGQPH FIRCIKPNDD
960 970 980 990 1000
RKALQFSQDR VLAQLRSTGI LETVSIRRQG YSHRIFFEEF VKRYYYLAFR
1010 1020 1030 1040 1050
AHQTPPANKE SCVAILEKSR LDHWVLGKTK VFLKYYHVEQ LNLLLREVMG
1060 1070 1080 1090 1100
RVVMLQAYTK GWLGARRYKR AKEKREKGAI TIQSAWRGYD ARRKLKQRSR
1110 1120 1130 1140 1150
RRSESEAHIH TVLQTTPDQK YCPDSGGESN RGHEETSRNC PAEADTDGHP
1160 1170 1180 1190 1200
QAQSPPTGCD VTSGHADTAA GYTVAELSVA GTDVSPSLVY HTASAHQRLS
1210 1220 1230 1240 1250
PCEDSLKPGS EEGLSQKQRA PRRRCQQPKM LSSPEDTMYY NQLNGTLEYQ
1260 1270 1280 1290 1300
GSQRKPRKLG QIKVLDGEDQ YYKCLSPGAC APEETHSVHP FFFSSSPRED

PFAQH
Length:1,305
Mass (Da):148,099
Last modified:December 16, 2008 - v2
Checksum:i9B8752F2704C7C80
GO
Isoform 2 (identifier: Q1EG27-2) [UniParc]FASTAAdd to basket
Also known as: Myosin IIIB2

The sequence of this isoform differs from the canonical sequence as follows:
     898-898: K → KKSPHSVPSYVLNTSPPK
     1149-1209: Missing.

Show »
Length:1,261
Mass (Da):143,762
Checksum:iDCCCFDBD868177A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti505E → K in AAX59998 (Ref. 1) Curated1
Sequence conflicti505E → K in AAX59999 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036028898K → KKSPHSVPSYVLNTSPPK in isoform 2. 1 Publication1
Alternative sequenceiVSP_0231121149 – 1209Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY830392 mRNA Translation: AAX59998.2
AY830393 mRNA Translation: AAX59999.2
AL845263, AL929164 Genomic DNA Translation: CAM21397.1
AL929164, AL845263 Genomic DNA Translation: CAM17979.1
RefSeqiNP_796350.2, NM_177376.4
XP_017174528.1, XM_017319039.1 [Q1EG27-1]
UniGeneiMm.99648

Genome annotation databases

EnsembliENSMUST00000112243; ENSMUSP00000107862; ENSMUSG00000042064 [Q1EG27-1]
GeneIDi329421
KEGGimmu:329421

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMYO3B_MOUSE
AccessioniPrimary (citable) accession number: Q1EG27
Secondary accession number(s): A2AR19, Q1EG26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 16, 2008
Last modified: March 28, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health