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Protein

Serine/threonine-protein kinase tousled-like 2

Gene

tlk2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of amino acid starvation-induced autophagy.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei417 – 4171ATPPROSITE-ProRule annotation
Active sitei518 – 5181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi394 – 4029ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase tousled-like 2By similarity (EC:2.7.11.1)
Alternative name(s):
PKU-alphaImported
Tousled-like kinase 2Imported
Gene namesi
Name:tlk2Imported
ORF Names:zgc:136697
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-060623-36. tlk2.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697Serine/threonine-protein kinase tousled-like 2PRO_0000367033Add
BLAST

Proteomic databases

PaxDbiQ1ECX4.

Expressioni

Gene expression databases

BgeeiQ1ECX4.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000003372.

Structurei

3D structure databases

ProteinModelPortaliQ1ECX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini388 – 666279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili265 – 29430Sequence analysisAdd
BLAST
Coiled coili336 – 37338Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1151. Eukaryota.
ENOG410Y3FX. LUCA.
GeneTreeiENSGT00790000123008.
HOGENOMiHOG000259522.
HOVERGENiHBG007938.
InParanoidiQ1ECX4.
KOiK08864.
OMAiQAEYHEQ.
OrthoDBiEOG78H3SF.
PhylomeDBiQ1ECX4.
TreeFamiTF315233.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR027086. TLK2.
[Graphical view]
PANTHERiPTHR22974:SF20. PTHR22974:SF20. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q1ECX4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEELHSLDP RRQELLEARF TGVGVAKGSG HNESSNQSLC SVGSLSDKEL
60 70 80 90 100
ETPEKKSNDQ RTRKRKGDPF DSQGKGGGRG HKISDYFEFA GGSGTGTSPA
110 120 130 140 150
RGIPPVARSS PQHSLSNPPA AVQQGSPSSI SSVNTDHSHT STSHKPASIH
160 170 180 190 200
TQHRASQSEL TMEKLAALES SKSSDLEKKE GRIDDLLRVN CDLRRQIDEQ
210 220 230 240 250
QKMLEWCKER LNKCVTMSKK LLIEKSKQEK IACREKSMQD RLRLGHFTTV
260 270 280 290 300
RHGASFTEQW TDGYAFQNLV KQQERVNGQR EEIERQRKLL IKKKPPSASQ
310 320 330 340 350
TPPPNLEPNK RKSKSNGAEN EMLSLAEYHE QEEIFKLRLG HLKKEEAEIQ
360 370 380 390 400
VELERLERVR NLHIRELKRI HNEDNSQFKD HPTLNDRYLL LHLLGRGGFS
410 420 430 440 450
EVYKAFDLTE QRYVAVKIHQ LNKNWRDEKK ENYHKHACRE YRIHKELDHP
460 470 480 490 500
RIVKLYDYFS LDTDSFCTVL EYCEGNDLDF YLKQHKLMSE KEARSIIMQV
510 520 530 540 550
VNALKYLNEI RPPIIHYDLK PGNILLVNGT ACGEIKITDF GLSKIMDDDN
560 570 580 590 600
YGVDGMELTS QGAGTYWYLP PECFVVGKEP PKISNKVDVW SVGVIFYQCL
610 620 630 640 650
YGKKPFGHNQ SQQDILQENT ILKATEVQFP PKPGVSPEAK AFIRRCLVYR
660 670 680 690
KEDRIDVHQL ASDPYLLPHI RKSVAATGNS SMAVASTSNS SNSSASN
Length:697
Mass (Da):79,305
Last modified:March 24, 2009 - v2
Checksum:i907B93FFE169E2E4
GO
Isoform 2 (identifier: Q1ECX4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-345: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:696
Mass (Da):79,175
Checksum:i956F6E35D4B8B9BD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401C → R in AAK52417 (Ref. 1) Curated
Sequence conflicti131 – 1311S → N in AAK52417 (Ref. 1) Curated
Sequence conflicti145 – 1451K → M in AAK52417 (Ref. 1) Curated
Sequence conflicti206 – 2061W → R in AAK52417 (Ref. 1) Curated
Sequence conflicti206 – 2061W → R in AAI54738 (Ref. 2) Curated
Sequence conflicti291 – 2911I → M in AAK52417 (Ref. 1) Curated
Sequence conflicti291 – 2911I → M in AAI54738 (Ref. 2) Curated
Sequence conflicti346 – 3461E → G in AAK52417 (Ref. 1) Curated
Sequence conflicti362 – 3621L → P in AAK52417 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei345 – 3451Missing in isoform 2. 1 PublicationVSP_053052

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF265344 mRNA. Translation: AAK52417.1.
BC117634 mRNA. Translation: AAI17635.1.
BC154737 mRNA. Translation: AAI54738.1.
BC171502 mRNA. Translation: AAI71502.1.
RefSeqiNP_001103887.1. NM_001110417.1.
XP_005163931.1. XM_005163874.2. [Q1ECX4-2]
UniGeneiDr.79789.

Genome annotation databases

EnsembliENSDART00000006490; ENSDARP00000003372; ENSDARG00000010779. [Q1ECX4-1]
ENSDART00000141937; ENSDARP00000114778; ENSDARG00000010779. [Q1ECX4-1]
GeneIDi407732.
KEGGidre:407732.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF265344 mRNA. Translation: AAK52417.1.
BC117634 mRNA. Translation: AAI17635.1.
BC154737 mRNA. Translation: AAI54738.1.
BC171502 mRNA. Translation: AAI71502.1.
RefSeqiNP_001103887.1. NM_001110417.1.
XP_005163931.1. XM_005163874.2. [Q1ECX4-2]
UniGeneiDr.79789.

3D structure databases

ProteinModelPortaliQ1ECX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000003372.

Proteomic databases

PaxDbiQ1ECX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000006490; ENSDARP00000003372; ENSDARG00000010779. [Q1ECX4-1]
ENSDART00000141937; ENSDARP00000114778; ENSDARG00000010779. [Q1ECX4-1]
GeneIDi407732.
KEGGidre:407732.

Organism-specific databases

CTDi11011.
ZFINiZDB-GENE-060623-36. tlk2.

Phylogenomic databases

eggNOGiKOG1151. Eukaryota.
ENOG410Y3FX. LUCA.
GeneTreeiENSGT00790000123008.
HOGENOMiHOG000259522.
HOVERGENiHBG007938.
InParanoidiQ1ECX4.
KOiK08864.
OMAiQAEYHEQ.
OrthoDBiEOG78H3SF.
PhylomeDBiQ1ECX4.
TreeFamiTF315233.

Miscellaneous databases

NextBioi20818585.
PROiQ1ECX4.

Gene expression databases

BgeeiQ1ECX4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR027086. TLK2.
[Graphical view]
PANTHERiPTHR22974:SF20. PTHR22974:SF20. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The zebrafish homolog of the human pKU-alpha protein kinase."
    Chou C.-M., Lee I.-L., Leu J.-H., Huang C.-J.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: ABImported.

Entry informationi

Entry nameiTLK2_DANRE
AccessioniPrimary (citable) accession number: Q1ECX4
Secondary accession number(s): A8WGJ6, Q90ZY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 11, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.