Q1EBK0 (SSN3_COCIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SSN3
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8

Gene names

Name:	SSN3
Synonyms:	CDK8
ORF Names:	CIMG_00063

Organism

Coccidioides immitis (strain RS) (Valley fever fungus) [Reference proteome]

Taxonomic identifier

246410 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides › Coccidioides immitis

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the SRB8-11 complex, a regulatory module of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Serine/threonine-protein kinase SSN3		PRO_0000312941
Regions
	Domain	79 – 408	330	Protein kinase
	Nucleotide binding	85 – 93	9	ATP By similarity
Sites
	Active site	211	1	Proton acceptor By similarity
	Binding site	109	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1EBK0 [UniParc]. Last modified January 9, 2013. Version 3. Checksum: 80730421A67B6106	FASTA	467	51,863
	10 20 30 40 50 60 MSFSNLPPSS GRGSHADGAS GRSMPPFPGS SSFTANNPSK GIHPNLTLKR SVQSFDESLD 70 80 90 100 110 120 NKKPLPFSYA SKVKLREKYL IVGFISSGTY GRVYKAKGRD GVGGDFAIKK FKPDKEGEKV 130 140 150 160 170 180 EYTGLSQSAI REISLCTELN HPNVVRLVET ILEDKCVYMV FEYTEHDLLQ IIHHHTQPQR 190 200 210 220 230 240 HAIPAPMVKS ILFQLLNGLL YLHSQWVMHR DLKPANILVT SKGAVRIGDL GLARVFKKPL 250 260 270 280 290 300 NSLFSGDKVV VTIWYRAPEL LLGARHYTTA VDLWAVGCIF AELLSLRPIF KGEEAKMDSK 310 320 330 340 350 360 KTVPFQRNQM LKIIEILGLP KKEKWPGLSS MPEFPQLQAM AMAPGSGHLH KPSNLEHWYH 370 380 390 400 410 420 VCLKSGGYSG SSPAGSPGKE GFDLLSRLLE YDPEKRISAK EALNHPYFTT GTPVAKNCFE 430 440 450 460 GFEGKYPNRR VSQDDNDIRS GSLPGTKRSG LPDDTLTSRA AKRAREM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.
[2]	"Population genomic sequencing of Coccidioides fungi reveals recent hybridization and transposon control." Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J., Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D., Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A., FitzGerald M. , Lui A., Macdonald J.P., Priest M., Orbach M.J., Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R., Taylor J.W., Rounsley S.D. Genome Res. 20:938-946(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: RS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG704911 Genomic DNA. Translation: EAS34670.2.
RefSeq	XP_001246253.1. XM_001246252.1.
3D structure databases
ProteinModelPortal	Q1EBK0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4567622.
KEGG	cim:CIMG_00024.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSN3_COCIM

Accession

Primary (citable) accession number: Q1EBK0
Secondary accession number(s): J3KG40

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program