Reviewed, UniProtKB/Swiss-Prot Q1EAR5 (CHI2_COCIM)

Names and origin

Protein names

Recommended name:
    Endochitinase 2
    EC=3.2.1.14

Gene names

Name:	CTS2
ORF Names:	CIMG_00348

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be associated with endosporulation.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Sequence caution	The sequence EAS34994.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 866	844	Endochitinase 2		PRO_0000252287
	Propeptide	867 – 895	29	Removed in mature form Potential		PRO_0000252288
Regions
	Compositional bias	346 – 840	495	Ser/Thr-rich
Amino acid modifications
	Lipidation	866	1	GPI-anchor amidated glycine Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1EAR5-1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: C34ECF3D2609C3CF	FASTA	895	94,380
	10 20 30 40 50 60 MGLTNILAAF IAVSSLFIQS LALNPYAKSN LAVYWGQGAG QNRLSYFCEK TSFDIIVVGF 70 80 90 100 110 120 INVFPDQGPA GWPGSNFGNQ CADSYYYTKN GTKTKLLDGC YQIKEDLPKC KALGKTILLS 130 140 150 160 170 180 LGGGAVHDFY EVKSEESALN FADFLWGAFG PLTPDWTGPR PFGEASVDGF DFDIEKGSNF 190 200 210 220 230 240 GYSIMVRRLR ELFLQDPLNR YYISAAPQCI MPDKYLSHAI SNSAFDFIFI QFYNNPSCSA 250 260 270 280 290 300 KRWVTNPKSV TYTVDDWVKY IRKSGNPLAK LFIGLPASKS AAAKEDYLTP GEATKIVSTY 310 320 330 340 350 360 MAKYPSTFGG MMVWEATASE NNKLGGLPYA DIMKEVLLRC DPDPPTSTVT STISASTSTQ 370 380 390 400 410 420 TSSQSTTMET KTLSASTTPS SPSTVSPSST MQTTSTGSTS TGTGTTSSQV TSSTTISTRS 430 440 450 460 470 480 ASTETVTTRS QEPPSTTIST RPASTETVTT RSQEPPSSTI STRSASTETV TTRSQEPPSS 490 500 510 520 530 540 TISTRSASTE TSTSSQDSPS TTISTKSAPT GTVTTRSQDL PSTTISTRSP ETETETVTTK 550 560 570 580 590 600 SQDSPSITLS TRSSSAETVS TRSQHSSSTT ISTKSAPTET GTTSEHSTSM PVSTRSASTE 610 620 630 640 650 660 TVITRSQNSD SQSMTVSTRS PSTESITTRS QGSPSETFST KSVPVDTIST ELPSQTHSTT 670 680 690 700 710 720 DSTPVSSSPT IPSGSTTIIP GTASDPVSAP TTTVPPNPTL TLAPSSSTTE DRTTITTIIT 730 740 750 760 770 780 TSYVTVCPTG FTTVTITYTT TYCPETASLT PTQAPIPGAP APPPDGWTTI VTVCPQCAPT 790 800 810 820 830 840 PTTVTLTVPT RSAFLPAPTE TRPVVTVVPV PENPIKNVKP SESGDFVTVT TVAPATVTKT 850 860 870 880 890 LEYNNPVDSD VNVQPTGGSS PVEFEGGAMT VRSMDVVAKA LITAGAAVLG LFLGL

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476726 Genomic DNA. Translation: EAS34994.1. Sequence problems.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.14. 97654.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_COCIM

Accession

Primary (citable) accession number: Q1EAR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)