Reviewed, UniProtKB/Swiss-Prot Q1E8M9 (PMIP_COCIM)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	CIMG_01084

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 795	773	Mitochondrial intermediate peptidase		PRO_0000338579
Sites
	Active site	562	1	By similarity
	Metal binding	561	1	Zinc; catalytic By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
	Metal binding	568	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1E8M9-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 168DA75ECA56482F	FASTA	795	89,923
	10 20 30 40 50 60 MLKTLNRRSW TCRQCIRILR RNAETRRYFQ GASAASPLCQ HTVSSSTSDK ARDDQTLRLI 70 80 90 100 110 120 FDSEPFWREF SQPKSSTSKR TGLLQNQYLT GPDGFLQFAQ VSLQKCQKIV AKVIAASTLE 130 140 150 160 170 180 DYRGMVRDLD RLSDLLCRVI DMAEFMKLNH PSPQIQDAAT QAYALMFEYM NVLNTTPELD 190 200 210 220 230 240 AQLKRASADL NVTSHWSPEE KVAARVLLKD FSQSAIHLPP KDRQKFVALS NEISQLGPMF 250 260 270 280 290 300 VTNRQPETDH VTVDKNKLRG MDPSLIQQLQ RWRKVAVPMF GDIPRIALYS VHDEETRKEI 310 320 330 340 350 360 YVTSRTSSKV QIRRLETLLQ KRAELAKLAG FPSYAHMTLS DKMAKTPEAV VNFLEALNAS 370 380 390 400 410 420 NRGQVQDELS QLLALKQADV PSATQLQPWD HAYYVHQYSA RHSRVRRSRE STLLPAFFSI 430 440 450 460 470 480 GTVIQGLSRL FTRLYGIRLV PTETLPGEIW NPDVRRLDVV DESDRRLAVI YCDLFTRPYK 490 500 510 520 530 540 SPNPTHFTLR GSREISQAEI AECADLSSSL HPNDGMATTI KPETNKLYQL PTVALICDFD 550 560 570 580 590 600 QSESRSTPSL LNEHNLETLF HEMGHAVHSV LARTDLQTIS GTRCATDFVE LPSVIMENFA 610 620 630 640 650 660 TAPEVLALYA RHWETNEPLP EHMVKSMELN RQSRVSMHGG MDNEVQILMA LLDQAYHSSR 670 680 690 700 710 720 PLEPNFDSTR IYHDVYSTHS SLPDPPGSRT SWQGYFAHLV GYGATYYSYL FDRAIANKVW 730 740 750 760 770 780 SDVFKGGELS TNRDAGERFK NEVLRWGGGR DGWNCVAGLL GNNPANDNGK LAEGGEEAMR 790 EVGRWGLGLM GTSEL

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476726 Genomic DNA. Translation: EAS35730.1.
RefSeq	XP_001247313.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	4567355.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_COCIM

Accession

Primary (citable) accession number: Q1E8M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)