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Q1E306 (ROK1_COCIM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase ROK1

EC=3.6.4.13
Gene names
Name:ROK1
ORF Names:CIMG_03057
OrganismCoccidioides immitis (strain RS) (Valley fever fungus) [Reference proteome]
Taxonomic identifier246410 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesCoccidioides

Protein attributes

Sequence length730 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes By similarity.

Subcellular location

Nucleusnucleolus By similarity.

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 730730ATP-dependent RNA helicase ROK1
PRO_0000256043

Regions

Domain228 – 442215Helicase ATP-binding
Domain482 – 650169Helicase C-terminal
Nucleotide binding241 – 2488ATP By similarity
Motif172 – 20433Q motif
Motif389 – 3924DEAD box

Sequences

Sequence LengthMass (Da)Tools
Q1E306 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: C7DBC691ACC18D98

FASTA73080,375
        10         20         30         40         50         60 
MDAFKLLTRS IKLKGSQLGS SSISSHLPSA GEANNPQLFP ASEADKTRGT KRKRESGEDT 

        70         80         90        100        110        120 
ELPNEIPVPD FFGSGSTAAE SPKAQRKRVT GKDADAEGQH GEETSMPEEE RKSILKSHKI 

       130        140        150        160        170        180 
KMTDLRTPPF RAVDTDISGK KSKRKKKKVE EPPPLTRKQL KAAQRLYPEP LTSFDKLRTR 

       190        200        210        220        230        240 
YHISRRLSDN IASQGYTVPT EVQLGSLPLL LGNAPAPNER TADDESLRSQ SEREPDLLVV 

       250        260        270        280        290        300 
APTGSGKTLS FMIPLINKIM KHHHDNPGLK EILAIVVAPT KELVAQIVNE GRKLTAGTGV 

       310        320        330        340        350        360 
KVSAVRKGMR IVEERGQETR SLEEENDGDD STASSDEEIH SSEGEKSKDI PLTKSDILVC 

       370        380        390        400        410        420 
TPLVLANALS DGGKRDVAPL PSVQKLVLDE ADVLLDPLFR EQTLSIWRAC THPQLRVGLW 

       430        440        450        460        470        480 
SATMGSNIEE LTKSTIKERQ ELLGLKDESS LIRLVVGLKD TAIPNISHKL VYAATEQGKL 

       490        500        510        520        530        540 
LGLRQLLHPT STSSSTTHLR PPFLIFTQTI ARAIALHSEL MYDIPAEAGG SSRIAVLHSE 

       550        560        570        580        590        600 
LSDSKRSDVM AGFRKGEIWV LITTDLLARG VDFRGINGVV NYDIPNSSAS YVHRVGRTGR 

       610        620        630        640        650        660 
AGREGGVAVT FYTKEDIPYV KNIANVISAS ENLRGTEEGE RIPKWLLDAL PSLSKNDKKD 

       670        680        690        700        710        720 
LKKYGVKARR PSTISDKTTS KKTRISTKSG FDRRMDNKRK GAIKGSQRRK AREQLNDEES 

       730 
EDDVWNGIDD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GG704916 Genomic DNA. Translation: EAS32118.2.
RefSeqXP_001243616.1. XM_001243615.1.
XP_001243701.1. XM_001243700.1.

3D structure databases

ProteinModelPortalQ1E306.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4563753.
4564331.
KEGGcim:CIMG_03057.
cim:CIMG_03142.

Phylogenomic databases

KOK01176.
K14779.
OrthoDBEOG74TX7R.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameROK1_COCIM
AccessionPrimary (citable) accession number: Q1E306
Secondary accession number(s): J3KAQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 11, 2006
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families