Reviewed, UniProtKB/Swiss-Prot Q1DZE0 (ATG15_COCIM)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	CIMG_04323

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Putative lipase ATG15		PRO_0000317962
Regions
	Topological domain	1	1	Cytoplasmic By similarity
	Transmembrane	2 – 22	21	Signal-anchor for type II membrane protein
	Topological domain	23 – 617	595	Lumenal By similarity
	Compositional bias	457 – 613	157	Thr-rich
Sites
	Active site	300	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	145	1	N-linked (GlcNAc...) Potential
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	559	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1DZE0-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 9B881ED735FC1134	FASTA	617	66,945
	10 20 30 40 50 60 MVACAIFLLL PIFSPVVFAT GLESHRNLQV PFVQPEDLGF LPQRSPQGHE FALRHIFHHG 70 80 90 100 110 120 TYQYPLLHKR LDVNPETKIW VASEDGAKLE FSPHFRVSSQ PSQIQRLTDR RVQVMEARLS 130 140 150 160 170 180 AARTPEVFST LSPPVWTIDE LPGPNISDKT TVLNLAKMTA NAYIMEPGAE RWEDVSGPFN 190 200 210 220 230 240 QSLSFGWEKD GLRGHIYADQ TNSTIIIALK GTSAALFDGD ETTTNDKIND NLFFSCCCGQ 250 260 270 280 290 300 GGQYFWRQVC DCYSSAYTCN STCLVSALVT ENRYYRASLD LYANVTDIYP NSTVWLAGHS 310 320 330 340 350 360 LGGAVSSLLG LTYGLPVVTF EGVPEALPAS RLGLPVPPGT DPNTPQAREY TGAYHFGHTA 370 380 390 400 410 420 DPVYMGTCNG ATSFCTLAGY AMESACHTGQ RCVYDTVGDL GWRVGIGTHR ILNVISDVIE 430 440 450 460 470 480 RYEDVPTCVP DTECRDCALW KFFESNGSHP IPPSSSTTSP TRTRTSTCET PGWWGCLDKT 490 500 510 520 530 540 TTTGTTTNVI TTSTSTCKTP GWFGCKDPTT TSSVPSATPS PTPIITSISM PTPTSTTCET 550 560 570 580 590 600 PGWFGGCNDP PTTATSKPNS SSPSTSTTCT RPGFFWGCWD PTSSSETPTA AFSSTATHTS 610 CASKTWFGRS CTTPAPR

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476727 Genomic DNA. Translation: EAS33299.1.
RefSeq	XP_001244882.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4565174.
Enzyme and pathway databases
BRENDA	3.1.1.3. 97654.
Family and domain databases
InterPro	IPR008262. Lipase_Ser_AS. [Graphical view]
PROSITE	PS00120. LIPASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_COCIM

Accession

Primary (citable) accession number: Q1DZE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)