Q1DXN1 (MCR1_COCIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
EC=1.6.2.2

Alternative name(s):
Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	CIMG_04932

Organism

Coccidioides immitis (strain RS) (Valley fever fungus)

Taxonomic identifier

246410 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.
Sequence caution	The sequence EAS33908.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	NADH-cytochrome b5 reductase 2		PRO_0000330179
Regions
	Transmembrane	32 – 48	17	Helical; Potential
	Domain	74 – 179	106	FAD-binding FR-type
	Nucleotide binding	182 – 217	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DXN1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: BDB2B6700DAA06FB	FASTA	325	36,509
	10 20 30 40 50 60 MFARFTFRTC RPAGQAIRKY ASEASPKPSS NVPLYGGLAL AAGGAYYYWQ RQQSPQALEA 70 80 90 100 110 120 ALKERSKVFI GGDQGWVDLK LAGIETLSHN VKRFRFEFPD SESVSGLHIA SALLTKYKGP 130 140 150 160 170 180 KDEKPTIRPY TPVSEEEQPG YLDLVVKQYP NGPMSTHLHN MAVGQQLSFK GPIPKYPWEQ 190 200 210 220 230 240 NKHDHICMIA GGTGITPMYQ IIRKIFNNPN DKTKVTLVFG NITEEDILLK KELDILENTY 250 260 270 280 290 300 PRRFRAFYLL DKPPAGWTQG TGYVTKELLK TVLPEPKTEN IKIFVCGPPG MYKAVSGPKN 310 320 SPKDQGELTG LLKELGYDKD QVYKF

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476727 Genomic DNA. Translation: EAS33908.1. Sequence problems.
RefSeq	XP_001245491.1. XM_001245490.1.
3D structure databases
ProteinModelPortal	Q1DXN1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4564902.
KEGG	cim:CIMG_04932.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_COCIM

Accession

Primary (citable) accession number: Q1DXN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	November 16, 2011
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program