Reviewed, UniProtKB/Swiss-Prot Q1DWN4 (NCB5R_COCIM)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	CIMG_05279

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	NADH-cytochrome b5 reductase 1		PRO_0000330150
Regions
	Transmembrane	10 – 27	18	Potential
	Domain	59 – 164	106	FAD-binding FR-type
	Nucleotide binding	144 – 159	16	FAD By similarity
	Nucleotide binding	170 – 207	38	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DWN4-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 3D322D70873DD664	FASTA	308	33,905
	10 20 30 40 50 60 MSSWTSKENI NGVYIPSALL IFGTTIIKKE WIAYATALAV VLSAWKLFSN KPRKVLNPTE 70 80 90 100 110 120 FQNFVLKDKT IVSHNVCIYR FALPRPTDIL GLPIGQHISL AATIPGQSKE IVRSYTPISS 130 140 150 160 170 180 DDDAGYFDLL VKSYPQGNIS KHLTTLRIGD KMKVRGPKGA MVYTPNMVRH IGMIAGGTGI 190 200 210 220 230 240 TPMLQVIKAI IKGRPRNGGN DTTQIDLIFA NVNPDDILLK EELDQLAKED DAFRIYYVLN 250 260 270 280 290 300 NPPEKWNGGV GFVTPDMIKA KLPAPAGDIK VLICGPPPMV SAMKKATESL GYKKANLVSK LEDQVFCF

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476727 Genomic DNA. Translation: EAS34255.1.
RefSeq	XP_001245838.1.
3D structure databases
SMR	Q1DWN4. Positions 51-297.
ModBase	Search...
Genome annotation databases
GeneID	4564615.
Phylogenomic databases
OrthoDB	EOG9KM0KS.
PhylomeDB	Q1DWN4.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_COCIM

Accession

Primary (citable) accession number: Q1DWN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 11, 2006
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)