Reviewed, UniProtKB/Swiss-Prot Q1DVD1 (LKHA4_COCIM)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

ORF Names:

CIMG_05732

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Leukotriene A-4 hydrolase		PRO_0000324927
Sites
	Active site	301	1	By similarity
	Active site	392	1	Proton donor By similarity
	Metal binding	300	1	Zinc; catalytic By similarity
	Metal binding	304	1	Zinc; catalytic By similarity
	Metal binding	323	1	Zinc; catalytic By similarity
	Site	204	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DVD1-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 91D14256BB6736F7	FASTA	621	70,941
	10 20 30 40 50 60 MATPANPLRD PNTLSNYNKF RTVHTSVNFE IRFDQKRLVG NVIHRLKSLT NAESKEVILD 70 80 90 100 110 120 SSYLEVKSVK VNGEAAEWQL LPRFEPYGSP LKISLEQAIP LDELIEVDIS VNTTEKCTAL 130 140 150 160 170 180 QWLNPEQTSN GKHPYMFSQC QAIHARAIFP CQDTPDVKAT FDFNLSSPLP VIASGVPVKQ 190 200 210 220 230 240 DASPPQSSGS IYYRFEQKVP IPSYLFAIAS GDIAQAQIGP RSHVAVSPDR LDECKWELEG 250 260 270 280 290 300 DTERFLQTIG NIIFPYVWGE YNVLILPPSF PYGGMENPVY TFATPSIISK DRQNVDVIAH 310 320 330 340 350 360 EISHSWSGNL VTNCSWEHFW LNEGWTTYLE RRVCLHCVFI CHGEPYRHFS AIIGWKHLVD 370 380 390 400 410 420 SVERHGDTHE FTKLVVDLKG KDPDDAFSSV PYEKGFTFIF HLENLIGKDK FDKFIPHYFT 430 440 450 460 470 480 RFRGKSLDSY EFKSCILDFF ASDEESHVLL NKLDWDSWFY KPGLPPKPSF DTSLVDVVYE 490 500 510 520 530 540 LANKWKYISQ SSFSPKASDM DGLVANQIVV FLEQVLLFDN PLTPEQSRFM GQVYNFAQSQ 550 560 570 580 590 600 NIEVSYLYLQ VGLKAGDDSI VEPTIKLLGE IGRMKFVRPL YRTLEKFNRD IAVDTFEKHK 610 620 NFYHPICRGL LEKDLFGDKG A

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476728 Genomic DNA. Translation: EAS30253.1. Different initiation.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.3.2.6. 97654.
Family and domain databases
InterPro	IPR012777. Leuk_A4_hydro_aminopept. IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_COCIM

Accession

Primary (citable) accession number: Q1DVD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)