Reviewed, UniProtKB/Swiss-Prot Q1DUF5 (CARA_COCIM)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase arginine-specific small chain
      Short name=CPS-A
    EC=6.3.5.5
Alternative name(s):
    Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	CPA1
ORF Names:	CIMG_06058

Organism

Coccidioides immitis [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290593
Regions
	Domain	225 – 412	188	Glutamine amidotransferase type-1
	Compositional bias	465 – 468	4	Poly-Ala
Sites
	Active site	301	1	Nucleophile By similarity
	Active site	385	1	By similarity
	Active site	387	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DUF5-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: AB825A81882BD468	FASTA	468	50,788
	10 20 30 40 50 60 MFSKALKSFP PRANWAMKPQ VLQARFMASV AKGTPKPNSA IFAPERATFT IRDGPVFHGK 70 80 90 100 110 120 SFGAKKNISG EAVFTTSLVG YPESLSDPSY RGQILVFTQP LIGNYGVPSA EKDENGLLKY 130 140 150 160 170 180 FESPSLQAVG VVVADVAEKY SHWTAVESLS EWCIREGVPA ISGVDTRAIV TYLREQGSSL 190 200 210 220 230 240 AKISVGEEYD ADQDEAFVDP EQINLVAKVS TKAPFHINPG NSNAHVAVID CGVKENILRS 250 260 270 280 290 300 LVHRGASITV FPYDFPIQKV AHHFDGVFIS NGPGDPTHCQ KTVHNLRKLM EVSQLPIMGI 310 320 330 340 350 360 CLGHQLLALA AGARTIKLKY GNRAHNIPAL DLTTGRCHIT SQNHGYAVDA STLPSDWKPY 370 380 390 400 410 420 FVNLNDSSNE GMIHKTRPIF STQFHPEAKG GPLDSSYLFD IYLDSVQKYK QSQAAFQPGR 430 440 450 460 DSRPSPLLAD LLGNERVGVQ TTIGTQSTPE SVAPVLETPI SPTMAAAA

References

[1]

"Annotation of the Coccidioides immitis RS genome." Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Zeng Q., Yandava C., Alvarado L., Oleary S., Orbach M.J., Galgiani J., Mandel M.A., Kellner E.M. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476728 Genomic DNA. Translation: EAS30579.1.
RefSeq	XP_001242162.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4561124.
Enzyme and pathway databases
BRENDA	6.3.5.5. 97654.
Family and domain databases
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_COCIM

Accession

Primary (citable) accession number: Q1DUF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	July 11, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)