Reviewed, UniProtKB/Swiss-Prot Q1DKE7 (DNLI4_COCIM)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
ORF Names:	CIMG_09216

Organism

Coccidioides immitis (Valley fever fungus) [Complete proteome]

Taxonomic identifier

5501 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 985	985	DNA ligase 4		PRO_0000278379
Regions
	Domain	711 – 804	94	BRCT 1
	Domain	878 – 983	106	BRCT 2
Sites
	Active site	312	1	N6-AMP-lysine intermediate By similarity
	Metal binding	379	1	Magnesium 1 Potential
	Metal binding	480	1	Magnesium 2 Potential
	Binding site	310	1	ATP By similarity
	Binding site	317	1	ATP By similarity
	Binding site	339	1	ATP By similarity
	Binding site	485	1	ATP By similarity
	Binding site	496	1	ATP By similarity
	Binding site	502	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DKE7-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: ADD8E6B21A345FEB	FASTA	985	112,847
	10 20 30 40 50 60 MDRLNNVGGE TERELDEKYP NRPRNKHSTL PFHDLFLTLF NPLNGNKKRP TGPAAARKKL 70 80 90 100 110 120 GPHGGQQTLS PQELRRDIIQ RFISRWRKEV GNDIYPAFRL IIPEKDRDRA MYGLKEKTIG 130 140 150 160 170 180 KLLVKIMKID KNSEDGFNLL NWKLPGQSMA SRMAGDFAGR CYEVISKRPI RTDVGNMTIQ 190 200 210 220 230 240 EVNDKLDVLA ATSKEDEQIP VLEEFYRNMN PEELMWLIRI ILRQMKVGAT ERTFFEIWHP 250 260 270 280 290 300 DAESLFSISS SLRRVCWELY DPNVRLEADE ARVTLMQCFQ PQLAQFQMHS FPKMIERMRL 310 320 330 340 350 360 SPDDPTFWIE EKLDGERIQL HMMSDDSIPG GKRFGFWSRK AKDYTYLYGN GFYDENGALT 370 380 390 400 410 420 RHLKDAFADG VDNIILDGEM ITWDPEQDAP LPFGTLKTAA LSEQRNPFSA TGQRPLFRIF 430 440 450 460 470 480 DILYLNDKAL TRYTLRDRRR ALEASIKPVH RRLEVHTYEI GNSAADIEPQ LRKVVAEASE 490 500 510 520 530 540 GLVLKNPNSP YRLNDRHDDW MKVKPEYMTE FGESLDCVVI GGYYGSGKRG GGLASFLCGL 550 560 570 580 590 600 RVDEAQVRQG ASPMKCYSFL KVGGGFTAPD YANIRHHTDG KWKDWNPKKP PTEFIELAGG 610 620 630 640 650 660 DAQYERPDVW IRPDESVVLC VKAASVTPSD QFRLGLTVRF PRFKRLRMDK DWKSALSIQE 670 680 690 700 710 720 FMDLKANAER EQKEKEFKID NSRRKRAKRA VKKPLTIAGY DESKRAGFTG PSGHVFEGMN 730 740 750 760 770 780 FFVITDSVEP EKKSKLELEQ LIKANGGKIY QTHTAAPNTL CIAERRTVKV ASVQKVAKES 790 800 810 820 830 840 IIRPSWLFDC IKQNEVDKGL PDLLIPFEPR HMYFTVKSQE EEIARHVDEY SDSYARDITP 850 860 870 880 890 900 NELSKLLVSM PLIPNLPPPH ISKTETQIQE RESTFQELRG WLFKNQVLHF VKRRSDSMLS 910 920 930 940 950 960 LPLRLASNLA RFAGASVANE LEDKSITHVV IDTDSQPADV SALRSAISKR VAAGRRIPHL 970 980 VTIAWIQDSW KAESLLDEER FAPTA

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476730 Genomic DNA. Translation: EAS28012.1.
RefSeq	XP_001239595.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4558489.
Enzyme and pathway databases
BRENDA	6.5.1.1. 97654.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 2 hits. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_COCIM

Accession

Primary (citable) accession number: Q1DKE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)