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Q1DCV9 (BIOB_MYXXD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MXAN_1257
OrganismMyxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]
Taxonomic identifier246197 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Biotin synthase HAMAP-Rule MF_01694
PRO_0000381490

Sites

Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1231Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1551Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2151Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2871Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1DCV9 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: 2721ABD454CC249B

FASTA34637,708
        10         20         30         40         50         60 
MPDTATVSSE SEFSGHAHVA APPPAGVDVR HDWSLSEVRA LYELPLLDLV HKAQTVHRAV 

        70         80         90        100        110        120 
FVDNKVQLCS LLSIKTGGCP EDCSYCPQAA RYKTGVKAEK LMAVPDVLDA ASKARAAGAT 

       130        140        150        160        170        180 
RFCMGAAWRE VKDGPQFDSV LEMVRGVRAL GMEACATLGM LSESQAKRLR EAGLSAYNHN 

       190        200        210        220        230        240 
LDTSPEHYGD IISTRTYEDR LRTLNRVRDA GISVCCGGII GMGESVDDRC NLLRTLANQE 

       250        260        270        280        290        300 
HHPESVPINA LVAVEGTPLQ EQQRVETVDM VRTIATARIL MPQSMVRLSA GRQQMNEEAQ 

       310        320        330        340 
LLCMMAGANS LFFGEKLLTT GNPEYTQDMA LLEKAGIRPL EPRQEG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000113 Genomic DNA. Translation: ABF89507.1.
RefSeqYP_629514.1. NC_008095.1.

3D structure databases

ProteinModelPortalQ1DCV9.
SMRQ1DCV9. Positions 30-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246197.MXAN_1257.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF89507; ABF89507; MXAN_1257.
GeneID4102439.
KEGGmxa:MXAN_1257.
PATRIC22645095. VBIMyxXan43560_1250.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMXAN246197:GIWU-1244-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYXXD
AccessionPrimary (citable) accession number: Q1DCV9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 11, 2006
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways