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Q1DCV9

- BIOB_MYXXD

UniProt

Q1DCV9 - BIOB_MYXXD

Protein

Biotin synthase

Gene

bioB

Organism
Myxococcus xanthus (strain DK 1622)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi123 – 1231Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi155 – 1551Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi215 – 2151Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi287 – 2871Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMXAN246197:GIWU-1244-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:MXAN_1257
    OrganismiMyxococcus xanthus (strain DK 1622)
    Taxonomic identifieri246197 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus
    ProteomesiUP000002402: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Biotin synthasePRO_0000381490Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246197.MXAN_1257.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1DCV9.
    SMRiQ1DCV9. Positions 30-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1DCV9-1 [UniParc]FASTAAdd to Basket

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    MPDTATVSSE SEFSGHAHVA APPPAGVDVR HDWSLSEVRA LYELPLLDLV    50
    HKAQTVHRAV FVDNKVQLCS LLSIKTGGCP EDCSYCPQAA RYKTGVKAEK 100
    LMAVPDVLDA ASKARAAGAT RFCMGAAWRE VKDGPQFDSV LEMVRGVRAL 150
    GMEACATLGM LSESQAKRLR EAGLSAYNHN LDTSPEHYGD IISTRTYEDR 200
    LRTLNRVRDA GISVCCGGII GMGESVDDRC NLLRTLANQE HHPESVPINA 250
    LVAVEGTPLQ EQQRVETVDM VRTIATARIL MPQSMVRLSA GRQQMNEEAQ 300
    LLCMMAGANS LFFGEKLLTT GNPEYTQDMA LLEKAGIRPL EPRQEG 346
    Length:346
    Mass (Da):37,708
    Last modified:July 11, 2006 - v1
    Checksum:i2721ABD454CC249B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000113 Genomic DNA. Translation: ABF89507.1.
    RefSeqiYP_629514.1. NC_008095.1.

    Genome annotation databases

    EnsemblBacteriaiABF89507; ABF89507; MXAN_1257.
    GeneIDi4102439.
    KEGGimxa:MXAN_1257.
    PATRICi22645095. VBIMyxXan43560_1250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000113 Genomic DNA. Translation: ABF89507.1 .
    RefSeqi YP_629514.1. NC_008095.1.

    3D structure databases

    ProteinModelPortali Q1DCV9.
    SMRi Q1DCV9. Positions 30-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246197.MXAN_1257.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF89507 ; ABF89507 ; MXAN_1257 .
    GeneIDi 4102439.
    KEGGi mxa:MXAN_1257.
    PATRICi 22645095. VBIMyxXan43560_1250.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci MXAN246197:GIWU-1244-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DK 1622.

    Entry informationi

    Entry nameiBIOB_MYXXD
    AccessioniPrimary (citable) accession number: Q1DCV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3