Q1DB00 (MASK_MYXXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase MasK
EC=2.7.10.2

Gene names

Name:	masK
Ordered Locus Names:	MXAN_1929

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus › Myxococcus xanthus

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for growth. Interacts with MglA to control social gliding motility.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with MglA. Ref.3
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Tyrosine-protein kinase MasK		PRO_0000282835
Regions
	Topological domain	1 – 415	415	Periplasmic Potential
	Transmembrane	416 – 433	18	Helical; Potential
	Topological domain	434 – 646	213	Cytoplasmic Potential
	Domain	25 – 300	276	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
	Compositional bias	456 – 539	84	Ala-rich
Sites
	Active site	163	1	Proton acceptor By similarity
	Binding site	57	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1DB00 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: C9667634284FE40C	FASTA	646	68,576
	10 20 30 40 50 60 MSPPQTTLPV TEAGLVPLLQ PYGPYVLVRK LAEGGMAEIF LAKLLGADGF ERNVVIKRML 70 80 90 100 110 120 PHLTNNPDFV EMFRDEARLA AKLAHPNIVQ IQELGFAEGC YYICMEYLAG EDFSTTLRLA 130 140 150 160 170 180 GRKRHYVPLP VVLRVLIDAA RGLHFAHEFT NEAGQPLNVV HRDISPSNLY LTYQGQVKVL 190 200 210 220 230 240 DFGIAKAESR LVNTRTGVVK GKYMYMAPEQ ARGKEVDRRA DIFALGVSLY EALTHVRPFS 250 260 270 280 290 300 RENDLAVLNA LLQGELKPPR ELRPDLPEEL EAILLKAMAF KPEDRYPTAE AFADALETFL 310 320 330 340 350 360 SEHLSGSGAM PLGAFLKGHF GEERFTERSR IPTLATLTAT YGGAAAGAQG QAPGAEPHGT 370 380 390 400 410 420 NLYGVLAREG DATSAQRPGM SMRPSSPGVP AHGAASRGST SPESAPTAGG RRWRTLAVGL 430 440 450 460 470 480 AGGLMLAAAG IVGYRQWMTT PASVSLVPAT VPVVEAVAPE AAAAQVGAPM EAVAPVGAAA 490 500 510 520 530 540 QAGSLTDAVA NGAGGDVGET DSAQLSVDAA GVTETDEAGL AGAASDVEAE ADEEGADAAP 550 560 570 580 590 600 VRSKKASSQK RVTLGIDDVQ RVVSRGRARI TTCFERYKAD LPSSQGEVQV QLTIVSSGKV 610 620 630 640 RAGTRGPLAS SGVGRCLEAQ AERLRFPPHR DQEVTVVMPF SWRVTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products." Stephens K., Hartzell P.L., Kaiser D. J. Bacteriol. 171:819-830(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DK 1622.
[3]	"MglA, a small GTPase, interacts with a tyrosine kinase to control type IV pili-mediated motility and development of Myxococcus xanthus." Thomasson B., Link J., Stassinopoulos A.G., Burke N., Plamann L., Hartzell P.L. Mol. Microbiol. 46:1399-1413(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH MGLA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF377950 Genomic DNA. Translation: AAK54653.1. CP000113 Genomic DNA. Translation: ABF90801.1.
RefSeq	YP_630173.1. NC_008095.1.
3D structure databases
ProteinModelPortal	Q1DB00.
ModBase	Search...
Protein-protein interaction databases
STRING	246197.MXAN_1929.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF90801; ABF90801; MXAN_1929.
GeneID	4105479.
KEGG	mxa:MXAN_1929.
PATRIC	22646407. VBIMyxXan43560_1900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
OMA	ICMEYLA.
ProtClustDB	CLSK2778165.
Enzyme and pathway databases
BioCyc	MXAN246197:GIWU-1911-MONOMER.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASK_MYXXD

Accession

Primary (citable) accession number: Q1DB00
Secondary accession number(s): Q93S46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program