Reviewed, UniProtKB/Swiss-Prot Q1D9V4 (PUR4_MYXXD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	MXAN_2349

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	1299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1299	1299	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264581
Regions
	Domain	1046 – 1299	254	Glutamine amidotransferase type-1
	Nucleotide binding	310 – 321	12	ATP Potential
Sites
	Active site	1139	1	Nucleophile By similarity
	Active site	1264	1	By similarity
	Active site	1266	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1D9V4-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: F9410F740D48DF96	FASTA	1,299	139,228
	10 20 30 40 50 60 MHTLRGAPAL SDFRLAKLLA QCRERVPSVS SIYAEFVHLI DAPAPLSEAD LATLGRLLDY 70 80 90 100 110 120 GPRVATGARI GSLLLVLPRP GTISPWSSKA TDIVHNCGLG ERVRRMERGT AFFIAGPDGQ 130 140 150 160 170 180 ALQDAELERL KPVLHDRMTQ AVVGRLEDAA ILFAGHTPRP FTTVDVLGGG RAALVTANRE 190 200 210 220 230 240 LGLALADDEM DYLVARFTEL KRNPTDVELM MFAQANSEHC RHKIFNASWT IDGKPQERSL 250 260 270 280 290 300 FQAIKNTYVQ HKEGVLGAYK DNAAVIEGFE VDRFFPSPES GEWGSVREPA HIMIKVETHN 310 320 330 340 350 360 HPTAISPYPG AATGAGGEIR DEGATGRGAR PKAGLTGFSV SHLRIPGFEQ PWEQPYGKPD 370 380 390 400 410 420 RIVSALDIMV DGPLGGAAFN NEFGRPNLTG YFRSYEVQVP TPGGVEVRGY HKPIMIAGGL 430 440 450 460 470 480 GNIRASHVQK GRLQPGDKLI VLGGPAMLIG LGGGAASSMA QGASAADLDF ASVQRDNAEM 490 500 510 520 530 540 ERRCQEVIDS CWALGDKNPI RSIHDVGAGG LSNAVPELAH DNDLGGRLEL RAVPNAEPGM 550 560 570 580 590 600 SPVEIWCNEA QERYVLGVAP EDLARFAALC ERERAPFSVL GDATAEQTLK LGDTQFGNAP 610 620 630 640 650 660 IDLPMDVLFG KPPRMHRDVT SRPVSFAPLT LDGSLALLAE RVLSHPTVAD KSFLITIGDR 670 680 690 700 710 720 TVSGLSSRDQ MVGPWQVPVA DCAVTLSTVT STTGEAMAMG ERTPLALIDA AASARMAVGE 730 740 750 760 770 780 ALTNIAAARI GKLSDVKLSA NWMAAAGSPG EDASLYAAVH AVGMELCPAL GLTIPVGKDS 790 800 810 820 830 840 MSMRTVWEEG GARKAVTSPV SLIISAFAPV LDVRKSLTPQ LVDVADDTRL LFIDLARGKQ 850 860 870 880 890 900 RLGGSVVAHV HGQVGPESPD VEDPALLRGF FAAVQALSES GSLLAYHDRS DGGLWATLCE 910 920 930 940 950 960 MAFAGRCGLD VDLAPLGGDV TAALFNEELG AVVQVRASDV ARVREVLAQH GLSRDVHELG 970 980 990 1000 1010 1020 RPVTALQVRV RHGGDTLMAE DTLALRRTWS RVSYEMQKLR DNPICADQES AARSDASDPG 1030 1040 1050 1060 1070 1080 LSPKLTFDPA QDVAAPFIAK GARPRVAVLR EQGVNSQQEM AAAFTRAGFA AVDVHMSDIL 1090 1100 1110 1120 1130 1140 SGRVSLEGFK GVLACGGFSY GDVLGAGGGW AKSILFNPRA RDAFAAFFAR PDSFGLGVCN 1150 1160 1170 1180 1190 1200 GCQMMSQLKD IIPGAEHFPR FVRNASEQYE ARLSLVEVSK TPSLFYQGME GSRMLIVTSH 1210 1220 1230 1240 1250 1260 GEGRAEFPSV EDAARVNGLG LVTTRWVDNH GRVAESYPAN PNGSPHGIAG LTTRDGRFTI 1270 1280 1290 TMPHPERVHR SVQHSWRPRE WGDDGPWMRM FRNARVWLG

References

[1]

"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed: 17015832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000113 Genomic DNA. Translation: ABF92728.1. Different initiation.
RefSeq	YP_630569.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1D9V4.
Genome annotation databases
GeneID	4107423.
GenomeReviews	Gene locus MXAN_2349 in contig CP000113_GR.
KEGG	mxa:MXAN_2349.
TIGR	MXAN_2349.
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG335309.
PhylomeDB	Q1D9V4.
Enzyme and pathway databases
BioCyc	MXAN246197:MXAN_2349-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_MYXXD

Accession

Primary (citable) accession number: Q1D9V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)