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Q1D8Y1 (SYE_MYXXD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MXAN_2675
OrganismMyxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]
Taxonomic identifier246197 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000330984

Regions

Motif11 – 2111"HIGH" region HAMAP-Rule MF_00022
Motif250 – 2545"KMSKS" region HAMAP-Rule MF_00022

Sites

Metal binding1081Zinc By similarity
Metal binding1101Zinc By similarity
Metal binding1351Zinc By similarity
Metal binding1371Zinc By similarity
Binding site2531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1D8Y1 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: A4EB8C0F73405E51

FASTA47953,209
        10         20         30         40         50         60 
MPPALRVRFA PSPTGYLHIG GARTALMNFL QARRQGGTFV LRMEDTDQGR STPESVQAIL 

        70         80         90        100        110        120 
DGLNWLGIDW DEGPGKEGPY APYFQMQRLD TYRKHADQLI AEGKAYRCYC TKEDLDAQRQ 

       130        140        150        160        170        180 
VAEKAGGAFK YPGTCRERTE PPAGRNAADA VIRFKMPAGD GSVSFTDKAL GTITKTHSDL 

       190        200        210        220        230        240 
DDWVMMRADG IPVYNFGCVI DDHLMDITLV ARGQEHVNST FPQLMLYQAL GWTPPDFAHL 

       250        260        270        280        290        300 
PLILGPDREK LSKRKHPEAD VMVHKRNGVM PEALLNFVIR LGWSHGNDEV ISREQMLEWF 

       310        320        330        340        350        360 
DFSDVGTTSG VWNPEKLLWL NQQWMKQLPV ETVVERLLPF LEAKGIQAKG DPRLETLVRT 

       370        380        390        400        410        420 
LRERSNTLED IATTAANVYF RSGITLDEKA ATKHLSGESL NLLRKVRETL TALPEWSVEA 

       430        440        450        460        470 
LDGVVKQVSE ASSVGMGKVA QPIRVALTGN TTSPGIGETL VLVGRDESLL RIDAALTRG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000113 Genomic DNA. Translation: ABF89409.1.
RefSeqYP_630892.1. NC_008095.1.

3D structure databases

ProteinModelPortalQ1D8Y1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246197.MXAN_2675.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF89409; ABF89409; MXAN_2675.
GeneID4103882.
KEGGmxa:MXAN_2675.
PATRIC22647885. VBIMyxXan43560_2627.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMAITESCRY.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycMXAN246197:GIWU-2652-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYXXD
AccessionPrimary (citable) accession number: Q1D8Y1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 11, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries