Reviewed, UniProtKB/Swiss-Prot Q1D359 (CHEB1_MYXXD)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	MXAN_4752

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264292
Regions
	Domain	8 – 123	116	Response regulatory
	Domain	151 – 344	194	CheB-type methylesterase
Sites
	Active site	163	1	By similarity
	Active site	190	1	By similarity
	Active site	286	1	By similarity
Amino acid modifications
	Modified residue	59	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1D359-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 7F8C2E0E34F72F77	FASTA	345	36,756
	10 20 30 40 50 60 MSSKGVISVL VIDDSAHNRR TLSTMLESEP DVRVVDRAAD GEEGLKKVVD LKPDVVTLDL 70 80 90 100 110 120 EMPKLGGYTF LRLLMRTAPT PVIVISSYSH RSDVFKALEL GAFDFIAKPQ HPTFEAMEAL 130 140 150 160 170 180 RVELLEKVRA ARHVRPGYRH AAPGARAMAV AGEPPLVVAV GASTGGPPAV QRVLEGLAVE 190 200 210 220 230 240 PTPCVLVCQH MPPQFTRAFA DRLDRIGPFS VTEARDGDLV QPGHVYIAPG GRHMVVAERG 250 260 270 280 290 300 SRLELQTPPP TPVDKYAPCV DRLFVSMAQV LGAKALGVVL TGMGADGAEG ARAVHRAGGE 310 320 330 340 VWAQSEETSV VFGMPGEAIA TGAVKRVIPL GDIGPALAAL ARRRR

References

[1]

"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed: 17015832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000113 Genomic DNA. Translation: ABF87312.1.
RefSeq	YP_632914.1.
3D structure databases
SMR	Q1D359. Positions 7-343.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q1D359. 7 interactions.
STRING	Q1D359.
Genome annotation databases
GeneID	4102107.
GenomeReviews	Gene locus MXAN_4752 in contig CP000113_GR.
KEGG	mxa:MXAN_4752.
TIGR	MXAN_4752.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	SQHMPAG.
PhylomeDB	Q1D359.
Enzyme and pathway databases
BioCyc	MXAN246197:MXAN_4752-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_MYXXD

Accession

Primary (citable) accession number: Q1D359

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 11, 2006
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)