Reviewed, UniProtKB/Swiss-Prot Q1CVR6 (T23O_MYXXD)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	MXAN_7400

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Tryptophan 2,3-dioxygenase		PRO_0000360119
Regions
	Region	24 – 28	5	Substrate binding By similarity
	Region	51 – 55	5	Substrate binding By similarity
Sites
	Metal binding	240	1	Iron (heme axial ligand) By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	124	1	Heme By similarity
	Binding site	254	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CVR6-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: A5ECE46C4AE3054A	FASTA	291	34,295
	10 20 30 40 50 60 MPGPMNKRDL EPGIITDLAG RTTYGDYLQL DRLLSAQVTR SQPPHHDELL FIIQHQTSEL 70 80 90 100 110 120 WMKLLIHELG ACIRYVQADR LEPSFKIFAR VAHIQRMLFE QWSVLETLTP NEYLEFRDTL 130 140 150 160 170 180 GSSSGFQSFQ YRAVEFLLGN KDAQALMPFR HVPAIHGELE RLFESPSLYD EFLRHLSRMG 190 200 210 220 230 240 HPVPQSHVQR DWRKPYEKSP EVVEVFRRIY QDAEAHWDAY EMCEKLVDTE ERFQLWRYRH 250 260 270 280 290 MMTVMRIIGF KQGTGGSSGV GFLRKALDLR FFPELWDVRT ELTPPPPRHR P

References

[1]

"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed: 17015832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000113 Genomic DNA. Translation: ABF86173.1.
RefSeq	YP_635507.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1CVR6.
Genome annotation databases
GeneID	4104959.
GenomeReviews	Gene locus MXAN_7400 in contig CP000113_GR.
KEGG	mxa:MXAN_7400.
TIGR	MXAN_7400.
Phylogenomic databases
HOGENOM	Q1CVR6.
OMA	MKLILHE.
Enzyme and pathway databases
BioCyc	MXAN246197:MXAN_7400-MON.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_MYXXD

Accession

Primary (citable) accession number: Q1CVR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)