Q1CUH2 (NADE_HELPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	HPAG1_0333

Organism

Helicobacter pylori (strain HPAG1) [Complete proteome] [HAMAP]

Taxonomic identifier

357544 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077560
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CUH2 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 99621F52F6C41679	FASTA	260	29,189
	10 20 30 40 50 60 MQKDYQKLIV YLCDFLEKEV QKKGFKKVVY GLSGGLDSAV VGVLCQKVFK ENAHALLMPS 70 80 90 100 110 120 SASMPENKTD ALSLCKKFSI SYTEYSIAPY DAIFGSHFKD ASLTRKGNFC ARLRMAFLYD 130 140 150 160 170 180 YSLKSDSLVI GTSNKSERML GYGTLFGDLA CAINPIGELF KTEVYELARR LNIPKKILNK 190 200 210 220 230 240 PPSADLFVGQ SDEKDLGYPY SVIDPLLKDI EALFKNKPID TETLTQLGYD EILVKNITSR 250 260 IQKNAFKLEL PTIAQKFNPK

References

[1]

"The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression." Oh J.D., Kling-Baeckhed H., Giannakis M., Xu J., Fulton R.S., Fulton L.A., Cordum H.S., Wang C., Elliott G., Edwards J., Mardis E.R., Engstrand L.G., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:9999-10004(2006) [PubMed: 16788065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HPAG1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000241 Genomic DNA. Translation: ABF84400.1.
RefSeq	YP_627074.1. NC_008086.1.
3D structure databases
ProteinModelPortal	Q1CUH2.
SMR	Q1CUH2. Positions 3-260.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1CUH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4098532.
GenomeReviews	Gene locus HPAG1_0333 in contig CP000241_GR.
KEGG	hpa:HPAG1_0333.
PATRIC	20601997. VBIHelPyl56048_0349.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	KLIVYLC.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	HPYL357544:HPAG1_0333-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HELPH

Accession

Primary (citable) accession number: Q1CUH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program