Reviewed, UniProtKB/Swiss-Prot Q1CTU5 (PYRC_HELPH)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	HPAG1_0560

Organism

Helicobacter pylori (strain HPAG1) [Complete proteome] [HAMAP]

Taxonomic identifier

357544 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Dihydroorotase		PRO_1000024017
Sites
	Metal binding	12	1	Zinc 1 By similarity
	Metal binding	14	1	Zinc 1 By similarity
	Metal binding	94	1	Zinc 1; via carbamate group By similarity
	Metal binding	94	1	Zinc 2; via carbamate group By similarity
	Metal binding	133	1	Zinc 2 By similarity
	Metal binding	167	1	Zinc 2 By similarity
	Metal binding	239	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	94	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CTU5-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: E8FC35F5706158CA	FASTA	339	38,044
	10 20 30 40 50 60 MEITLFDPID AHLHVRENAL LKAVLRYSSE PFSAAVIMPN LSKPLIDTPT TLEYEEEILK 70 80 90 100 110 120 NSSNFKPLMS LYFNDDLTLE ELQRAHEKGV RFLKLYPKGM TTNAQNGTSD LLDEKTLEIL 130 140 150 160 170 180 ENAQKLGFIL CIHAEQAGFC LDKEFLCHSV LETFALSFPK LKIIIEHLSD WRSIALIEKH 190 200 210 220 230 240 DNLYATLTLH HISMTLDDLL GGSLNPHCFC KPLIKTKKDQ ERLLSLALKA HPKISFGSDS 250 260 270 280 290 300 APHFVSKKHS ANIPAGIFSA PILLPALCEL FEKHNALENL QAFISNNAKT IYGLDNLPSK 310 320 330 KARLSKKPFI VPTHALCLNE KIAILRGGET LSWNLQEIA

References

[1]

"The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression." Oh J.D., Kling-Baeckhed H., Giannakis M., Xu J., Fulton R.S., Fulton L.A., Cordum H.S., Wang C., Elliott G., Edwards J., Mardis E.R., Engstrand L.G., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 103:9999-10004(2006) [PubMed: 16788065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000241 Genomic DNA. Translation: ABF84627.1.
RefSeq	YP_627301.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4099416.
GenomeReviews	Gene locus HPAG1_0560 in contig CP000241_GR.
KEGG	hpa:HPAG1_0560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1CTU5.
Enzyme and pathway databases
BioCyc	HPYL357544:HPAG1_0560-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006992. Amidohydro_2. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF04909. Amidohydro_2. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HELPH

Accession

Primary (citable) accession number: Q1CTU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)