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Q1CTC3 (TRMB_HELPH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HPAG1_0732
OrganismHelicobacter pylori (strain HPAG1) [Complete proteome] [HAMAP]
Taxonomic identifier357544 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288158

Sites

Binding site1241S-adenosyl-L-methionine By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1761S-adenosyl-L-methionine By similarity
Binding site2321Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1CTC3 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: CC3EA4912DE0FD2C

FASTA39345,707
        10         20         30         40         50         60 
MPHFLAKLDF KPLKYPLIEG DFCFHKEFLS LKNPTKSCVY ASFKDRIFLL QKIRRANDFL 

        70         80         90        100        110        120 
IKSEKTTPLK REILKQALRI YSQSFEVISH NLQENSKHAS GKKALDLGTF EDFIQKNQAP 

       130        140        150        160        170        180 
ILAEIGFGSG RHLIELAKNN PTKTCLGIEI HTPSIAQALK QIELLDLKNL HILQGDGRLI 

       190        200        210        220        230        240 
LESMPNYKCE KIFVHFPVPW NEKKHRRVLS EKFLNEALRV LKPRGFLELR TDDGLYFEDS 

       250        260        270        280        290        300 
LKLALKNFQC EIEIKKNAQI PVISKYEARW NKLKKDIYDL RIYSLELNET PFYNHAFDFS 

       310        320        330        340        350        360 
FDTITMSEKS VGTILKTKKI IQEGYFVHVC NIYENKGDFL VELSMGDFDW PVRLFVLLTE 

       370        380        390 
NQIFYLNKSP LKTLNNHKAH LLLQNILSQK GIG 

« Hide

References

[1]"The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression."
Oh J.D., Kling-Baeckhed H., Giannakis M., Xu J., Fulton R.S., Fulton L.A., Cordum H.S., Wang C., Elliott G., Edwards J., Mardis E.R., Engstrand L.G., Gordon J.I.
Proc. Natl. Acad. Sci. U.S.A. 103:9999-10004(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HPAG1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000241 Genomic DNA. Translation: ABF84799.1.
RefSeqYP_627473.1. NC_008086.1.

3D structure databases

ProteinModelPortalQ1CTC3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357544.HPAG1_0732.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF84799; ABF84799; HPAG1_0732.
GeneID4098820.
KEGGhpa:HPAG1_0732.
PATRIC20602825. VBIHelPyl56048_0760.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000252163.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHPYL357544:GH1F-743-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HELPH
AccessionPrimary (citable) accession number: Q1CTC3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 11, 2006
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways