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Q1CRK7

- SYI_HELPH

UniProt

Q1CRK7 - SYI_HELPH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter pylori (strain HPAG1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi913 – 9131ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHPYL357544:GH1F-1384-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HPAG1_1348
    OrganismiHelicobacter pylori (strain HPAG1)
    Taxonomic identifieri357544 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000008835: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 920920Isoleucine--tRNA ligasePRO_1000022078Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi357544.HPAG1_1348.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1CRK7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiTALWQIT.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1CRK7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQVFKR MQNRKDNHGD    50
    FTLHDGPPYA NGHLHLGHAL NKILKDIVVK REYFKGNKIY YTPGWDCHGL 100
    PIEQQILERL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD 150
    FEDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE 200
    VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL 250
    KKDAIYALTQ KGYLVAKALH EKLAALGVVD SEIAHEFNAN DLEYLKASNP 300
    LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLRY NLEVLMSVDE 350
    KGCYDEGIIH NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPY 400
    CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALD AIEKVEFVPS 450
    SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA 500
    HLFEKKGCDV WWESSVKDLL PPSYQEDATH YEKVMHILDV WFDSGSTFKA 550
    VLEDYHGEKG QSPSDVILEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH 600
    GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ 650
    TFFIQTEQHY KKFRNTLKFL LANFSDMDLK NLERSHNFSP LDHFILEALE 700
    TISVGVNSAF EEHDFVKGLN VLMAFVTNEL SGIYLDACKD SLYCDSKNNE 750
    KRQAIQMVLL ATASKLCYFL APILTHTIEE VLEHSQVLCA FLQAKDVFDL 800
    KDISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE GVIKNSLECA 850
    IEVKEKALRE NLVEELLMVS FVGIAKEKLS ETPAFTLFKA PFYKCPRCWR 900
    FKSELENTPC KRCEEVLKER 920
    Length:920
    Mass (Da):106,023
    Last modified:July 11, 2006 - v1
    Checksum:i1F622080AA183E28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000241 Genomic DNA. Translation: ABF85415.1.
    RefSeqiYP_628089.1. NC_008086.1.

    Genome annotation databases

    EnsemblBacteriaiABF85415; ABF85415; HPAG1_1348.
    GeneIDi4098165.
    KEGGihpa:HPAG1_1348.
    PATRICi20604150. VBIHelPyl56048_1396.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000241 Genomic DNA. Translation: ABF85415.1 .
    RefSeqi YP_628089.1. NC_008086.1.

    3D structure databases

    ProteinModelPortali Q1CRK7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 357544.HPAG1_1348.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF85415 ; ABF85415 ; HPAG1_1348 .
    GeneIDi 4098165.
    KEGGi hpa:HPAG1_1348.
    PATRICi 20604150. VBIHelPyl56048_1396.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi TALWQIT.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HPYL357544:GH1F-1384-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression."
      Oh J.D., Kling-Baeckhed H., Giannakis M., Xu J., Fulton R.S., Fulton L.A., Cordum H.S., Wang C., Elliott G., Edwards J., Mardis E.R., Engstrand L.G., Gordon J.I.
      Proc. Natl. Acad. Sci. U.S.A. 103:9999-10004(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HPAG1.

    Entry informationi

    Entry nameiSYI_HELPH
    AccessioniPrimary (citable) accession number: Q1CRK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3