SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q1CRK7

- SYI_HELPH

UniProt

Q1CRK7 - SYI_HELPH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, HPAG1_1348
Organism
Helicobacter pylori (strain HPAG1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity
Metal bindingi913 – 9131Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPYL357544:GH1F-1384-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HPAG1_1348
OrganismiHelicobacter pylori (strain HPAG1)
Taxonomic identifieri357544 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000008835: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligaseUniRule annotationPRO_1000022078Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi357544.HPAG1_1348.

Structurei

3D structure databases

ProteinModelPortaliQ1CRK7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTALWQIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CRK7-1 [UniParc]FASTAAdd to Basket

« Hide

MEEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQVFKR MQNRKDNHGD    50
FTLHDGPPYA NGHLHLGHAL NKILKDIVVK REYFKGNKIY YTPGWDCHGL 100
PIEQQILERL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD 150
FEDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE 200
VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL 250
KKDAIYALTQ KGYLVAKALH EKLAALGVVD SEIAHEFNAN DLEYLKASNP 300
LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLRY NLEVLMSVDE 350
KGCYDEGIIH NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPY 400
CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALD AIEKVEFVPS 450
SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA 500
HLFEKKGCDV WWESSVKDLL PPSYQEDATH YEKVMHILDV WFDSGSTFKA 550
VLEDYHGEKG QSPSDVILEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH 600
GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ 650
TFFIQTEQHY KKFRNTLKFL LANFSDMDLK NLERSHNFSP LDHFILEALE 700
TISVGVNSAF EEHDFVKGLN VLMAFVTNEL SGIYLDACKD SLYCDSKNNE 750
KRQAIQMVLL ATASKLCYFL APILTHTIEE VLEHSQVLCA FLQAKDVFDL 800
KDISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE GVIKNSLECA 850
IEVKEKALRE NLVEELLMVS FVGIAKEKLS ETPAFTLFKA PFYKCPRCWR 900
FKSELENTPC KRCEEVLKER 920
Length:920
Mass (Da):106,023
Last modified:July 11, 2006 - v1
Checksum:i1F622080AA183E28
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000241 Genomic DNA. Translation: ABF85415.1.
RefSeqiYP_628089.1. NC_008086.1.

Genome annotation databases

EnsemblBacteriaiABF85415; ABF85415; HPAG1_1348.
GeneIDi4098165.
KEGGihpa:HPAG1_1348.
PATRICi20604150. VBIHelPyl56048_1396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000241 Genomic DNA. Translation: ABF85415.1 .
RefSeqi YP_628089.1. NC_008086.1.

3D structure databases

ProteinModelPortali Q1CRK7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357544.HPAG1_1348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF85415 ; ABF85415 ; HPAG1_1348 .
GeneIDi 4098165.
KEGGi hpa:HPAG1_1348.
PATRICi 20604150. VBIHelPyl56048_1396.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi TALWQIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HPYL357544:GH1F-1384-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression."
    Oh J.D., Kling-Baeckhed H., Giannakis M., Xu J., Fulton R.S., Fulton L.A., Cordum H.S., Wang C., Elliott G., Edwards J., Mardis E.R., Engstrand L.G., Gordon J.I.
    Proc. Natl. Acad. Sci. U.S.A. 103:9999-10004(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HPAG1.

Entry informationi

Entry nameiSYI_HELPH
AccessioniPrimary (citable) accession number: Q1CRK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi