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Q1CMV1

- SYI_YERPN

UniProt

Q1CMV1 - SYI_YERPN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYPES377628:GIXK-379-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:YPN_0347
ORF Names:YP516_0354
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000008936: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000022145Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi377628.YPN_0347.

Structurei

3D structure databases

ProteinModelPortaliQ1CMV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CMV1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRAAKKGKKT
60 70 80 90 100
FILHDGPPYA NGNIHIGHSV NKILKDIIVK SKGMAGYDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRTACRKYA AEQVEGQKKD FIRLGVLGDW
160 170 180 190 200
DHPYLTMDFK TEANIIRALS KIIDNGHLHK GAKPVHWCTD CGSSLAEAEV
210 220 230 240 250
EYYDKTSQSI DVRFNAVDTA TVAAKFGVSA VNGPISLVIW TTTPWTLPAN
260 270 280 290 300
RAISLNAEYL YQLVQVEGEC LILAADLVES VMKRAGITQW AVLGSCTGSD
310 320 330 340 350
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDFVIGQKYG
360 370 380 390 400
LEVANPVGPN GCYLAGTYPT LDGLFVFKAN DVVVELLREK GALLHVEKLL
410 420 430 440 450
HSYPCCWRHK TPIIFRATPQ WFISMDQKGL RKQSLQEIKG VQWIPDWGQA
460 470 480 490 500
RIETMVANRP DWCISRQRTW GVPMSLFVHK ETEQLHPRSI ELMEEVAKRV
510 520 530 540 550
EQDGIQAWWD LDPAEILGAD AADYVKVPDT LDVWFDSGST HSSVVDVRPE
560 570 580 590 600
FGGHSPDMYL EGSDQHRGWF MSSLMIATAM KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT ISPQDVMNKL GGDILRLWVA STDYTGEIAV SDEILKRSAD
660 670 680 690 700
SYRRIRNTAR FLLANLNGFD PAQHQVKPEE MVVVDRWAVG RAQAAQAEIM
710 720 730 740 750
EAYENYDFHL VVQRLMQFCS VEMGSFYLDI IKDRQYTAKG DGIARRSCQT
760 770 780 790 800
ALFHIAEALV RWMAPIMSFT ADEIWNHLPG ERQQYVFTEE WYDGLFGLAG
810 820 830 840 850
NESMNDTFWA ELLKVRGEVN KVLEQARSDK RIGGSLEAAV TLYAEPELAA
860 870 880 890 900
RLNSLQDELR FVLLTSAAKV AAYADAGNDA QQSELIAGLK ITFNKADGEK
910 920 930
CPRCWHYTQD VGLVAEHAEL CGRCVTNVAG DGEERKFA
Length:938
Mass (Da):104,690
Last modified:July 11, 2006 - v1
Checksum:i827A66208EE0B84A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG16679.1.
ACNQ01000006 Genomic DNA. Translation: EEO78132.1.
RefSeqiYP_646279.1. NC_008149.1.

Genome annotation databases

EnsemblBacteriaiABG16679; ABG16679; YPN_0347.
EEO78132; EEO78132; YP516_0354.
GeneIDi4123335.
KEGGiypn:YPN_0347.
PATRICi18599683. VBIYerPes46733_0526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG16679.1 .
ACNQ01000006 Genomic DNA. Translation: EEO78132.1 .
RefSeqi YP_646279.1. NC_008149.1.

3D structure databases

ProteinModelPortali Q1CMV1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 377628.YPN_0347.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG16679 ; ABG16679 ; YPN_0347 .
EEO78132 ; EEO78132 ; YP516_0354 .
GeneIDi 4123335.
KEGGi ypn:YPN_0347.
PATRICi 18599683. VBIYerPes46733_0526.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci YPES377628:GIXK-379-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_YERPN
AccessioniPrimary (citable) accession number: Q1CMV1
Secondary accession number(s): C4GNQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3