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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi270Manganese 2UniRule annotation1
Metal bindingi275Manganese 1UniRule annotation1
Metal bindingi275Manganese 2UniRule annotation1
Active sitei282UniRule annotation1
Metal bindingi293Manganese 2UniRule annotation1
Metal bindingi352Manganese 1UniRule annotation1
Metal bindingi354Manganese 1UniRule annotation1
Metal bindingi354Manganese 2UniRule annotation1
Active sitei356UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:YPN_0647
ORF Names:YP516_0683
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000008936 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000200051 – 503Probable cytosol aminopeptidaseAdd BLAST503

Structurei

3D structure databases

ProteinModelPortaliQ1CM01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000243132.
KOiK01255.
OMAiMPPNVAN.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CM01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG
60 70 80 90 100
ELEGKVGQTL LLHHVPNILS ERILLIGCGK ERELDERQYK QVIQKTINTL
110 120 130 140 150
NDTGSMEAVC FLTELHVKGR NTYWKVRQAV ETAKETLYTF DQLKSNKTEP
160 170 180 190 200
RRPLRKMVFN VPTRRELTSG ERAIQHGLAI ASGIKAAKDL GNMPPNICNA
210 220 230 240 250
AYLASQARQL ADAFSTNTVT RVIGEQQMKE LGMHAYLAVG HGSQNESLMS
260 270 280 290 300
VIEYKGNPNK DAKPIVLVGK GLTFDSGGIS IKPAEGMDEM KYDMCGAATV
310 320 330 340 350
YGVMRVVAEL QLPLNVVGVL AGCENMPGGR AYRPGDILTT MSGQTVEVLN
360 370 380 390 400
TDAEGRLVLC DALTYVERFE PELVIDIATL TGACVVALGN HLTGLMSNHN
410 420 430 440 450
PLAHELIGAS EQAGDRAWRL PLGEEYYEQL DSNFADMANI GGRAGGAITA
460 470 480 490 500
GCFLSRFTRK YSWAHLDIAG TAWRSGKNKG ATGRPVALLS QFLLNRAGLN

GDD
Length:503
Mass (Da):54,797
Last modified:July 11, 2006 - v1
Checksum:iE3A27876304218F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG16979.1.
ACNQ01000006 Genomic DNA. Translation: EEO78446.1.
RefSeqiWP_002209310.1. NZ_ACNQ01000006.1.

Genome annotation databases

EnsemblBacteriaiABG16979; ABG16979; YPN_0647.
EEO78446; EEO78446; YP516_0683.
KEGGiypn:YPN_0647.
PATRICi18600353. VBIYerPes46733_0861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG16979.1.
ACNQ01000006 Genomic DNA. Translation: EEO78446.1.
RefSeqiWP_002209310.1. NZ_ACNQ01000006.1.

3D structure databases

ProteinModelPortaliQ1CM01.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG16979; ABG16979; YPN_0647.
EEO78446; EEO78446; YP516_0683.
KEGGiypn:YPN_0647.
PATRICi18600353. VBIYerPes46733_0861.

Phylogenomic databases

HOGENOMiHOG000243132.
KOiK01255.
OMAiMPPNVAN.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_YERPN
AccessioniPrimary (citable) accession number: Q1CM01
Secondary accession number(s): C4GPL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.