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Q1CM01 (AMPA_YERPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:YPN_0647
ORF Names:YP516_0683
OrganismYersinia pestis bv. Antiqua (strain Nepal516) [Complete proteome] [HAMAP]
Taxonomic identifier377628 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_1000020005

Sites

Active site2821 Potential
Active site3561 Potential
Metal binding2701Manganese 2 By similarity
Metal binding2751Manganese 1 By similarity
Metal binding2751Manganese 2 By similarity
Metal binding2931Manganese 2 By similarity
Metal binding3521Manganese 1 By similarity
Metal binding3541Manganese 1 By similarity
Metal binding3541Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1CM01 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: E3A27876304218F2

FASTA50354,797
        10         20         30         40         50         60 
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG ELEGKVGQTL 

        70         80         90        100        110        120 
LLHHVPNILS ERILLIGCGK ERELDERQYK QVIQKTINTL NDTGSMEAVC FLTELHVKGR 

       130        140        150        160        170        180 
NTYWKVRQAV ETAKETLYTF DQLKSNKTEP RRPLRKMVFN VPTRRELTSG ERAIQHGLAI 

       190        200        210        220        230        240 
ASGIKAAKDL GNMPPNICNA AYLASQARQL ADAFSTNTVT RVIGEQQMKE LGMHAYLAVG 

       250        260        270        280        290        300 
HGSQNESLMS VIEYKGNPNK DAKPIVLVGK GLTFDSGGIS IKPAEGMDEM KYDMCGAATV 

       310        320        330        340        350        360 
YGVMRVVAEL QLPLNVVGVL AGCENMPGGR AYRPGDILTT MSGQTVEVLN TDAEGRLVLC 

       370        380        390        400        410        420 
DALTYVERFE PELVIDIATL TGACVVALGN HLTGLMSNHN PLAHELIGAS EQAGDRAWRL 

       430        440        450        460        470        480 
PLGEEYYEQL DSNFADMANI GGRAGGAITA GCFLSRFTRK YSWAHLDIAG TAWRSGKNKG 

       490        500 
ATGRPVALLS QFLLNRAGLN GDD 

« Hide

References

[1]"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nepal516.
[2]"Yersinia pestis Nepal516A whole genome shotgun sequencing project."
Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000305 Genomic DNA. Translation: ABG16979.1.
ACNQ01000006 Genomic DNA. Translation: EEO78446.1.
RefSeqYP_646579.1. NC_008149.1.

3D structure databases

ProteinModelPortalQ1CM01.
SMRQ1CM01. Positions 1-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING377628.YPN_0647.

Proteomic databases

PRIDEQ1CM01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG16979; ABG16979; YPN_0647.
EEO78446; EEO78446; YP516_0683.
GeneID4125286.
KEGGypn:YPN_0647.
PATRIC18600353. VBIYerPes46733_0861.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243132.
KOK01255.
OMAANEAKMS.
OrthoDBEOG6FV8B3.

Enzyme and pathway databases

BioCycYPES377628:GIXK-682-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_YERPN
AccessionPrimary (citable) accession number: Q1CM01
Secondary accession number(s): C4GPL4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries