Q1CM01 (AMPA_YERPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	YPN_0647
ORF Names:	YP516_0683

Organism

Yersinia pestis bv. Antiqua (strain Nepal516) [Complete proteome] [HAMAP]

Taxonomic identifier

377628 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pestis

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Probable cytosol aminopeptidase HAMAP-Rule MF_00181		PRO_1000020005
Sites
	Active site	282	1	Potential
	Active site	356	1	Potential
	Metal binding	270	1	Manganese 2 By similarity
	Metal binding	275	1	Manganese 1 By similarity
	Metal binding	275	1	Manganese 2 By similarity
	Metal binding	293	1	Manganese 2 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	354	1	Manganese 1 By similarity
	Metal binding	354	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CM01 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: E3A27876304218F2	FASTA	503	54,797
	10 20 30 40 50 60 MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG ELEGKVGQTL 70 80 90 100 110 120 LLHHVPNILS ERILLIGCGK ERELDERQYK QVIQKTINTL NDTGSMEAVC FLTELHVKGR 130 140 150 160 170 180 NTYWKVRQAV ETAKETLYTF DQLKSNKTEP RRPLRKMVFN VPTRRELTSG ERAIQHGLAI 190 200 210 220 230 240 ASGIKAAKDL GNMPPNICNA AYLASQARQL ADAFSTNTVT RVIGEQQMKE LGMHAYLAVG 250 260 270 280 290 300 HGSQNESLMS VIEYKGNPNK DAKPIVLVGK GLTFDSGGIS IKPAEGMDEM KYDMCGAATV 310 320 330 340 350 360 YGVMRVVAEL QLPLNVVGVL AGCENMPGGR AYRPGDILTT MSGQTVEVLN TDAEGRLVLC 370 380 390 400 410 420 DALTYVERFE PELVIDIATL TGACVVALGN HLTGLMSNHN PLAHELIGAS EQAGDRAWRL 430 440 450 460 470 480 PLGEEYYEQL DSNFADMANI GGRAGGAITA GCFLSRFTRK YSWAHLDIAG TAWRSGKNKG 490 500 ATGRPVALLS QFLLNRAGLN GDD

References

[1]	"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nepal516.
[2]	"Yersinia pestis Nepal516A whole genome shotgun sequencing project." Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000305 Genomic DNA. Translation: ABG16979.1. ACNQ01000006 Genomic DNA. Translation: EEO78446.1.
RefSeq	YP_646579.1. NC_008149.1.
3D structure databases
ProteinModelPortal	Q1CM01.
SMR	Q1CM01. Positions 1-503.
ModBase	Search...
Protein-protein interaction databases
STRING	377628.YPN_0647.
Proteomic databases
PRIDE	Q1CM01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG16979; ABG16979; YPN_0647. EEO78446; EEO78446; YP516_0683.
GeneID	4125286.
KEGG	ypn:YPN_0647.
PATRIC	18600353. VBIYerPes46733_0861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HOG000243132.
KO	K01255.
OMA	LMSVMEY.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17.
InterPro	IPR011356. Leucine_aapep/pepB. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_leu_NH2_pept. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. PTHR11963:SF3. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_YERPN

Accession

Primary (citable) accession number: Q1CM01
Secondary accession number(s): C4GPL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program