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Q1CLS2

- CYSG1_YERPN

UniProt

Q1CLS2 - CYSG1_YERPN

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Protein

Siroheme synthase 1

Gene

cysG1

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei247 – 2471Proton acceptorUniRule annotation
Active sitei269 – 2691Proton donorUniRule annotation
Binding sitei305 – 3051S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei382 – 3821S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei411 – 4111S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADUniRule annotation
Nucleotide bindingi43 – 442NADUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYPES377628:GIXK-761-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1UniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1UniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylase 1UniRule annotation
Alternative name(s):
SUMT 1UniRule annotation
Uroporphyrinogen III methylase 1UniRule annotation
Short name:
UROM 1UniRule annotation
Precorrin-2 dehydrogenase 1UniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelatase 1UniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysG1UniRule annotation
Ordered Locus Names:YPN_0726
ORF Names:YP516_0774
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000008936: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Siroheme synthase 1PRO_0000330574Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281PhosphoserineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi377628.YPN_0726.

Structurei

3D structure databases

ProteinModelPortaliQ1CLS2.
SMRiQ1CLS2. Positions 1-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
BLAST
Regioni215 – 472258Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
BLAST
Regioni300 – 3023S-adenosyl-L-methionine bindingUniRule annotation
Regioni330 – 3312S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CLS2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYLPLFADL KQRPVLIVGG GEVAARKIEL LHRAGAQVWV VAQTLSSELE
60 70 80 90 100
QQYQDGRIHW LAQDFLPEQL DNVFLVIAAT NDTVLNAAVF AAADQRCILA
110 120 130 140 150
NVVDDQPLCS FIFPSIVDRS PLVVAISSSG QAPVLARILR EKLEALLPTR
160 170 180 190 200
LSDMAAIAGR WRGRVKQHMA SMGERRRFWE HAFSGRFASL ISRGQLTEAE
210 220 230 240 250
NELQLSLEGQ HRALGEVALV GAGPGDAGLL TLRGLQVMQQ ADVVLYDHLV
260 270 280 290 300
SPEVLDLVRR DAERICVGKR AGAHSVTQEA TNQLLVTLAQ QGKRVVRLKG
310 320 330 340 350
GDPFIFGRGG EELQVVAQAG IPFQVVPGVT AAAGATAYAG IPLTHRDHAQ
360 370 380 390 400
SVTFITGHCR PDGDDLDWQT LARGRQTLAI YMGTVKAAAI SQQLIAHGRS
410 420 430 440 450
STTPVAVIGR GTRVDQQVLI GTLAQLESLA QQAPTPALLV IGEVVNLHHQ
460 470
IAWFGQQPQT ESAISPSVVN LA
Length:472
Mass (Da):50,904
Last modified:July 11, 2006 - v1
Checksum:i1BD7DB7E5ADE5222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG17058.1.
ACNQ01000007 Genomic DNA. Translation: EEO77922.1.
PIRiAI0408.
RefSeqiYP_646658.1. NC_008149.1.

Genome annotation databases

EnsemblBacteriaiABG17058; ABG17058; YPN_0726.
EEO77922; EEO77922; YP516_0774.
GeneIDi4123560.
KEGGiypn:YPN_0726.
PATRICi18600544. VBIYerPes46733_0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG17058.1 .
ACNQ01000007 Genomic DNA. Translation: EEO77922.1 .
PIRi AI0408.
RefSeqi YP_646658.1. NC_008149.1.

3D structure databases

ProteinModelPortali Q1CLS2.
SMRi Q1CLS2. Positions 1-456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 377628.YPN_0726.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG17058 ; ABG17058 ; YPN_0726 .
EEO77922 ; EEO77922 ; YP516_0774 .
GeneIDi 4123560.
KEGGi ypn:YPN_0726.
PATRICi 18600544. VBIYerPes46733_0955.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi LHQQLAW.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci YPES377628:GIXK-761-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiCYSG1_YERPN
AccessioniPrimary (citable) accession number: Q1CLS2
Secondary accession number(s): C4GPR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 11, 2006
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3